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一年生黑麦草制取燃料酒精发酵工艺的初步研究
引用本文:代,郭和蓉,张兴龙,李强. 一年生黑麦草制取燃料酒精发酵工艺的初步研究[J]. 草业学报, 2012, 21(1): 220-228. DOI: 10.11686/cyxb20120129
作者姓名:代  郭和蓉  张兴龙  李强
作者单位:华南农业大学农学院,广东广州,510642
基金项目:广东省科技计划项目(工业攻关计划;能源技术),广东软科学计划项目
摘    要: 以分别经过酸预处理(1% H2SO4)和碱预处理(10% NaOH),再用绿色木酶酶解的一年生黑麦草邦德(Abundant)为材料,进行了燃料酒精发酵工艺研究。结果表明,通过预处理后,方案1(普通酵母+毕赤酵母,体积比7∶3)的相对最优发酵条件为:温度37℃,菌株浓度10%,pH5.5,时间48h。方案2(普通酵母+毕赤酵母+黑曲霉,体积比7∶3∶2)的相对最优发酵条件为:温度37℃,菌株浓度12%,pH5.5,时间48h。2种方案中,菌株浓度和发酵温度是比较重要的影响因素。对于酸碱预处理而言,均是发酵方案2 的原料酒精转化率相对较高,分别为8.36%和8.39%,差异不显著。

关 键 词:一年生黑麦草  发酵工艺  酒精度  原料酒精转化率  燃料酒精

A preliminary study on fermentation technology in the manufacture of fuel alcohol using annual ryegrass
DAI Ying , GUO He-rong , ZHANG Xing-long , LI Qiang. A preliminary study on fermentation technology in the manufacture of fuel alcohol using annual ryegrass[J]. Acta Prataculturae Sinica, 2012, 21(1): 220-228. DOI: 10.11686/cyxb20120129
Authors:DAI Ying    GUO He-rong    ZHANG Xing-long    LI Qiang
Affiliation:(Agricultural Science College,South China Agricultural University,Guangzhou 510642,China)
Abstract:The fermentation technology of fuel alcohol manufacturing transformation was studied using an annual ryegrass(Lolium multiflorum) variety for abundant material.It was pretreated with 1% H2SO4 and 10% NaOH then hydrolyzed by Trichoderma viride.The relative optimum conditions of program 1(a volume ratio of Saccharomyces cerevisiae to Pichia pastoris of 7∶3) were a 10% concentration of the yeasts at 37℃,pH 5.5 for 48 h.For program 2(a ratio of S.cerevisiae,P.pastoris,and Aspergullus niger of 7∶3∶2) a 12% concentration of the mixture at 37℃,pH 5.5 was tested for 48 h.The concentration of strains and the fermentation temperature were the more important factors in both programs.For pretreatments with H2SO4 and NaOH,program 2 achieved relatively higher alcohol conversion rates(8.36% and 8.39%,respectively) but there was no significant difference.
Keywords:annual ryegrass(Lolium multiflorum)  fermentation technology  alcohol content  alcohol conversion rate of raw material  fuel alcohol
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