| 小麦中杂色曲霉毒素的硅镁吸附柱层析分离纯化及HPLC的定量分析 |
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| 引用本文： | 袁建,杜娟.小麦中杂色曲霉毒素的硅镁吸附柱层析分离纯化及HPLC的定量分析[J].粮食储藏,2010,39(6):40-43. |
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| 作者姓名： | 袁建 杜娟 |
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| 作者单位： | 南京财经大学食品科学与工程学院,江苏南京210003 |
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| 基金项目： | 国家"十一五"科技支撑项目 |
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| 摘 要： | 目的:建立小麦中杂色曲霉毒素含量的高效液相色谱检测方法。方法:样品中的杂色曲霉毒素经正己烷提取后,采用国产硅镁型吸附剂制备层析柱净化后,运用高效液相色谱法测定,色谱柱为Nova-Pak C18柱,以甲醇-水(70∶30,V/V)为流动相,流速0.7mL/min,检测波长为246nm进行检测。结果:该方法平均加标回收率为79.26%,相对标准偏差为3.84%,最低检出量为0.017μg/kg。结论:本方法简便、准确、成本低,可用于小麦中杂色曲霉毒素含量的测定。
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| 关 键 词： | 高效液相色谱 杂色曲霉毒素 硅镁型吸附剂 净化 |
DETERMINATION OF STERIGMATOCYSTIN IN WHEAT BY HPLC WITH FLORISIL COLUMN |
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| Yuan Jian,Du Juan.DETERMINATION OF STERIGMATOCYSTIN IN WHEAT BY HPLC WITH FLORISIL COLUMN[J].Grain Storage,2010,39(6):40-43. |
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| Authors: | Yuan Jian Du Juan |
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| Institution: | Yuan Jian Du Juan(School of Food Science and Technology,Nanjing University of Finance and Economics 210003) |
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| Abstract: | HPLC method for the determination of sterigmatocystin in grains was developed.Sterigmatocystin was extracted by hexane and cleaned-up using florisil column.Analysis was carried out by using Nova-Pak C18 column with an isocratic mobile phase composed of methanol-water（70∶30,V/V）,flow speed 0.7 mL/m and UV detection at 246 nm.Results： The average recovery was 79.26% with the RSD of 3.84%,limit of detection was 0.017 μg/kg.Conclusion： The method is simple,accurate,cheaper,and can be used for the determination of sterigmatocystin in wheat. |
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| Keywords: | HPLC sterigmatocystin magnesium trisilicat purification |
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