| 转基因甜瓜植株的获得及其抗病性 |
| |
| 引用本文： | 王慧中,赵培洁,周晓云.转基因甜瓜植株的获得及其抗病性[J].植物保护学报,2000,27(2):126-130. |
| |
| 作者姓名： | 王慧中 赵培洁 周晓云 |
| |
| 作者单位： | [1]杭州师范学院生物系 [2]浙江大学 |
| |
| 基金项目： | 浙江省自然科学基金(393105)，杭州市人才基金(98083) |
| |
| 摘 要： | 甜瓜（Ｃｕｃｕｍｉｓ ｍｅｌｏ）子叶外植体能被含双元载体的根瘤农杆菌菌不妨功体内含１个ＮＰＴ－Ⅱ基因（新霉素磷酸基转移酶Ⅱ基因），以供选择卡妹妹纱抗性的转化甜 １个ＷＭＶ－２ ＣＰ基因（西瓜花叶病毒２号外壳蛋白基因）。卡那霉素抗性植株经ＰＣＲ－Ｓｏｕｔｈｅｒｎ杂交试验表明，外源的ＷＭＶ－２ ＣＰ基因确已通过农杆菌介导的转化途径导入甜瓜细胞。目前转基因甜瓜已开花结果。攻毒试验表明，与对照相比，转基因
|
| 关 键 词： | 甜瓜 根瘤农杆菌 转基因植株 抗病性 WWM－2基因 |
REGENERATION OF TRANSGENIC CUCUMIS MELO AND ITS RESISTANCE TO VIRUS DISEASES |
| |
| Wang Huizhong,Zhao Peijie and Zhou Xiaoyun.REGENERATION OF TRANSGENIC CUCUMIS MELO AND ITS RESISTANCE TO VIRUS DISEASES[J].Acta Phytophylacica Sinica,2000,27(2):126-130. |
| |
| Authors: | Wang Huizhong Zhao Peijie and Zhou Xiaoyun |
| |
| Institution: | Department of Biology, Hangzhou Normal College, Hangzhou 310036,College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029 and Department of Bioengineering, Zhejiang University of Technology, Hangzhou 310032 |
| |
| Abstract: | Segments of cotyledon explants was infected by Agrobacterium tumefaciens harboring a binary vector that contains a NPT-II gene and a WMV-2 CP gene. Transgenic plants were obtained. Successful genetic transformation was confirmed by PCR-Southem blot. The results of the resistance test of transgenic Cucumis melo to WMV-2 show that transgenic plants can delay the disease infection and reduce the incidence of virus diseases, thus showing certain resistance to relevant virus. |
| |
| Keywords: | Cucumis melo Agrobacterium tumefaciens tiansfonnation transgenic plants |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
| 点击此处可从《植物保护学报》下载免费的PDF全文 |
