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| 提高农杆菌介导法将PinII抗虫基因导入滇型水稻的转化效率 | |
| 引用本文： | 曾黎琼,丁玉梅,段玉云,董广,字映生,黄兴奇,程在全.提高农杆菌介导法将PinII抗虫基因导入滇型水稻的转化效率[J].分子植物育种,2004,2(1):19-24. |
| 作者姓名： | 曾黎琼 丁玉梅 段玉云 董广 字映生 黄兴奇 程在全 |
| 作者单位： | 1. 云南省农科院生物技术研究所,昆明,650223 2. 玉溪市农科所,玉溪,653100 3. 云南大学生命学院生物系,昆明,650091 |
| 基金项目： | 本研究由云南省国际合作项目资助(项目编号:99C003)基金项目资助 |
| 摘 要： | 本论文对滇型水稻品种玉优一号、HT-7的愈伤组织诱导、基因转化及植株再生进行了研究。通过实验，将质粒pCAMBI1300上带有的PinⅡ抗虫基因导入水稻愈伤组织细胞中，获得了一批Hyg抗性植株。185株抗性苗经过PCR检测和叶片对潮霉素的抗性试验，证明其中有59株为转基因阳性植株。结果显示，受体、农杆菌状态以及共培养方式等是影响基因转化效率的关键因素；以叶片对潮霉素的抗性作为转基因植株的快速筛选是可行的，大大提高了检测效率。 |
| 关 键 词： | 农杆菌介导法 PinⅡ抗虫基因 滇型水稻 转基因 共培养 |
Improving the Transgenic Frequency of the Gene PinII Mediated by Agrobacterium tumefaciens in Yunnan Rice |
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| Zeng Liqiong Ding Yumei Duan Yuyun Dong Guang Zi Yingsheng Huang Xingqi Cheng Zaiquan Institute of Biotechnology,of Yunan Academy of Agricultural Sciences,Kunming, Yuxi Institue of Agricultural Sciences,Yuxi, College of Life Sciences,Yunnan University,Kunming.Improving the Transgenic Frequency of the Gene PinII Mediated by Agrobacterium tumefaciens in Yunnan Rice[J].Molecular Plant Breeding,2004,2(1):19-24. | |
| Authors: | Zeng Liqiong Ding Yumei Duan Yuyun Dong Guang Zi Yingsheng Huang Xingqi Cheng Zaiquan Institute of Biotechnology of Yunan Academy of Agricultural Sciences Kunming Yuxi Institue of Agricultural Sciences Yuxi College of Life Sciences Yunnan University Kunming |
| Institution: | Zeng Liqiong Ding Yumei 1 Duan Yuyun 1 Dong Guang 2 Zi Yingsheng 3 Huang Xingqi Cheng Zaiquan 1* 1 Institute of Biotechnology,of Yunan Academy of Agricultural Sciences,Kunming,650223 2 Yuxi Institue of Agricultural Sciences,Yuxi,653100 3 College of Life Sciences,Yunnan University,Kunming,650091 |
| Abstract: | The material used in this experiment was Yunnan rice varieties Yuyou1 and HT-7.The calli induction, gene transformation and plant regeneration were carried out in the paper. The gene PinII in plasmid pCAMBI1300 was transferred into the cell of rice calli, some resistant plants were obtained by screened in hygromycin selecting culture.185 plantlets were used to be indentified by PCR and assayed leaf resistance to hygomycin, 59 plantlets of them were positive. The results showed that the status of receptor and Agrobacterium tumefaciens and co-culturing were the influencing factors in rice gene transformation, The Bio-assay of leaf resistance to hygomycin would be very effective in the selection of rice transgenic plants. |
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