甘蓝型油菜含油量及皮壳率的QTL分析

【目的】通过构建甘蓝型油菜遗传连锁图谱，对含油量及皮壳率进行QTL分析。【方法】以黄籽亲本GH06和黑籽亲本P174杂交得到的F2:6家系的188个株系为作图群体，利用SRAP、SSR、AFLP及TRAP四种标记构建遗传连锁图谱，在此基础上采用复合区间作图法（CIM）对含油量及皮壳率两个性状进行QTL分析。【结果】图谱包含19个连锁群、300个标记位点，总长为1 248.5 cM。共得到7个与含油量相关的QTL，单位点遗传贡献率在3.73%～10.46%之间；4个与皮壳率相关的QTL，单位点遗传贡献率在4.89%～6.84%之间。【结论】黄籽油菜具有高含油量的优势；皮壳率对含油量有显著影响；SRAP标记具有较好的检测QTL位点的能力。

QTL Analysis of Oil and Hull Content in Brassica napus L

Abstract：【Objective】Constructing the linkage map of Brassica napus L. and mapping the QTL of oil content and hull content. 【Method】F2:6 RIL population with 188 lines, crossed by GH06 as female parent and Youyan 2 as male parent，were used as mapping Population. The SRAP、SSR、AFLP and TRAP markers were used to construct the linkage map, and the composite interval mapping (CIM) was used to identify quantitative trait loci associated with oil content and hull content.【Result】300 markers were integrated into 19 groups with software JoinMap3.0. It covered 1248.5cM with a mean marker interval of 4.16cM. Eight QTL were found for oil content with the phenotypic variance explained of 5.95％～9.47％; Two for hull content with the phenotypic variance explained of 6.91％and 6.05％respectively. 【Conclusion】It was confirmed that SRAP marker was a good marker system for the construction of linkage map. The hull content has a great effect on the oil content.