家兔暴发病毒性出血症的病理学诊断

对送检的9只家兔进行病理解剖,对心血、肝、脾及肠系膜淋巴结进行细菌分离培养,并选取心、肝、脾、肺等组织按常规病理制片进行病理组织学检查,应用免疫组化染色(ABC法)检测肝细胞内兔病毒性出血症病毒(RHDV)抗原,结果证实此次家兔自然暴发的传染病即是兔病毒性出血症(RHD)。其临床主要表现为最急性型及急性型,病理变化特点以全身微循环障碍为主;DIC形成,以实质器官淤血、水肿、出血、变性、坏死为主要特点,其中以肝、肾、脾等器官病变较重,尤其肝脏的病变具有特征性。综合病理学检测结果,确定此次家兔暴发的是RHD。

Pathological Diagnosis on An Outbreak of Rabbits ViralHemorrhagic Disease

Nine rabbits were pathologically dissected and their hearts,livers,spleens and mesenteric lymph nodes were used to isolate bacteria.And the hearts,livers,spleens,lungs were selected to prepared histological section for histopathologic examination.The immunohistochemical staining method(ABC method) with RHDV antigen was used to find RHDV antigen in the cells of livers.So the disease was diagnosed RHD.The clinical characters were mainly the most acute and the acute type and the pathological changes were microcirculation disturbance in the whole body.DIC formed and the congestion,edema,hemorrhage,degeneration,necrosis were observed in the parenchymal organs,and severe in the liver,kidney and spleen,especially very severe in the liver.The results confirmed that the rabbit outbreaks of disease was of RHD.