甘肃中部地区豌豆根腐病病原研究

在豌豆根腐病病原研究中,采用了PDA、WA组织分离、胡萝卜诱饵分离及水培养分离等不同的分离技术,共分离病株820株,病组织1734块,获得菌株235个,经初步鉴定确定为17个不同的种,对这些分离物采用灭菌土盆栽、蛭石盆栽、纸卷法、断根法等接种技术进行致病性测定。根据分离率和致病性测定结果,认为发生在甘肃中部地区的豌豆根腐病是由茄镰刀菌(Fusarium solani(Mars)Sacc)、豌豆丝囊霉(Aphanomyces euteiches)、链孢粘帚霉(Gliocladium catenulatum Gilman et Abbott)、尖孢镰刀菌(Fusarium oxysporum Schlecht)、根串珠霉(Thielaviopsis basicola)、立枯丝核菌(Rhizoctonia solani Kuhn)、腐霉(Pythium spp)和壳二孢菌(Ascochyta spp)等复合侵染引起的。根据文献记载,上述各类菌除茄镰刀菌、尖孢镰刀菌和丝囊霉之外,其余真菌在豌豆上的致病性国内未见报道,链孢粘帚霉作为豌豆根腐病的致病菌为国内外首次报道。

Study on the Pathogens of Root Rot of Pea in the Central Region of Gansu Province

In this paper, the pathogens of pea root rot from Dinxi, Tongwei counties were studied with different isolating media such as PDA, WA, carrot bait, water culture. Two hundred and thirty five isolates were obtained from 1734 pieces of 820 plants of diseased peas. After purification, pathogenic test, reisolation and identification, the 235 isolates belong to seventeen species of 11 genera. According to the identification results of isolating rate and pathogenicity, Fusarium solani(Mart.)Sacc, Aphanomyces euteiches, Thielaviopsis basicola, Gliocladium catenulatum Gilman et Abbott Rhizoctonia solani Kuhn, Fusarium oxysporum Schlecht, Pythium spp. and Ascochyta spp. are main components of pea root rot complex. In addition to Fusarium solani, Fusarium oxysporum and Aphanomyces euteiches, the rest of them that have pathogenicity on peas are new records in China.