| 新疆哈密瓜上两种病毒的血清学特性 |
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| 引用本文： | 尹玉琦,崔星明,向本春,刘仪,梁训生.新疆哈密瓜上两种病毒的血清学特性[J].植物保护学报,1985,12(4):231-236. |
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| 作者姓名： | 尹玉琦 崔星明 向本春 刘仪 梁训生 |
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| 作者单位： | 中国农科院植物保护研究所 |
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| 摘 要： | 铜绿金龟(虫甲)是北京地区的一种重要害虫,一年发生一代,以3龄幼虫在土壤51～75厘米深层越冬。成虫每年6～9月出现,成虫有较强的趋光性,一日内以19:30′～21:00′活动最盛。成虫食性杂,每一雌虫一生可产卵30～40粒。产卵期为4～5天。在9种含水量(0.3、2、4、6、8、10、15、20和25%)土壤与5种温度(10、17、25、30、37℃)组合下,试验结果卵在温度25℃与8～15%含水量土壤内孵化率最高。卵的发育起点温度是9.14±1.15℃,发育有效积温为178.05日度。幼虫季节性垂直分布与10厘米土温变化有关,秋季当10厘米土温低于10℃,幼虫向土壤深层迁移,春季当10厘米土温高于6℃幼虫向土壤表层迁移。每年4～5月及8～9月幼虫10厘米作物外进行为害,这时亦导防治的有利时机。
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SEROLOGYCAL DISTINCTION OF TWO VIRUS INFECTING SACCHARINE MELON IN XINJIANG |
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| Yin Yuqi,Cui Xinming,Xiang Bencun,Lui Yi and Liang Xuesheng.SEROLOGYCAL DISTINCTION OF TWO VIRUS INFECTING SACCHARINE MELON IN XINJIANG[J].Acta Phytophylacica Sinica,1985,12(4):231-236. |
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| Authors: | Yin Yuqi Cui Xinming Xiang Bencun Lui Yi and Liang Xuesheng |
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| Institution: | Xinjiang Shihezi Agricultural College,Xinjiang Shihezi Agricultural College,Xinjiang Shihezi Agricultural College,Beijing Agricultural Univercity and Beijing Agricultural Univercity |
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| Abstract: | The writers have made purification and prepared antiserum for two viruses (WMV-2,and CMV) isolated from saccharine melon in Xinjiang from 1982-1983. Cucumber mosaic virus (CMV) was purified by using clarification of expressed infected tobacco leaf sap in 0.5 M pH 6.6 citrate buffer containing 0.1% 2-ME with 1/2 chloroform and two cycles of differential centrifugation,prepared for antiserum of CMV. |
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