果蝇唾腺染色体的制作技术研究

目的]为果蝇唾腺染色体试验教学及相关研究提供成熟的制作技术。方法]以野生型果蝇三龄幼虫为试验材料,通过三龄幼虫培养、唾腺剥离、压片、解离、染色等完整的制片过程探索染色体制片规律。结果]利用镊子柄部进行压片效果很好,染色体伸展充分。通过比较4种染色试剂（苏木精、醋酸洋红、卡宝品红和改良苯酚品红）的染色效果选出的最佳试剂为改良苯酚品红。结论]该研究为果蝇唾腺染色体的制作提供了成熟的技术参考。

Observation Technique on Drosophila Salivary Gland Chromosome

Objective]The aim of this study was to provide mature manual technique for experiment teaching of Drosphila salivary gland chromosome and other related research.Method]The three-days larvas of wild Drosphila were took as experiment materials.The making rule of temporary chromosome specimen was explored throughout the complete course.Result]By comparison,the staining effect of modified phenol fuchsion was much better than other conventional methods.Conclusion]This study has provided the mature technical ...