茶花鸡体尺性状和屠宰性能的测定及相关性分析

ttttt目的ttttt从国家级畜禽资源保护品种茶花鸡中分离乳酸菌，研究其益生特性，以期为禽用益生菌制剂研发奠定基础。ttttttttttttt方法ttttt采集散养茶花鸡新鲜粪便，使用MRS培养基分离乳酸菌，采用生理生化试验和16S rDNA基因序列分析法鉴定，并研究其胃肠道耐受性、抑菌活性、抗生素敏感性和肠上皮细胞黏附能力等益生特性。ttttttttttttt结果ttttt分离得到CHF4-1、CHF6-2、CHF7-2和CHF10-2共4株菌株。生理生化鉴定和16S rDNA序列分析结果表明：CHF4-1、CHF6-2、CHF7-2和CHF10-2分别为唾液乳杆菌、加氏乳杆菌、罗伊氏乳杆菌和约氏乳杆菌；与参考菌株鼠李糖乳杆菌GG相比，CHF6-2和CHF10-2具有较强的胃肠道耐受性和抗菌活性；加氏乳杆菌CHF6-2除对头孢噻肟中度敏感之外，对其余9种抗生素均敏感，且其细胞黏附水平为(13.07±0.22)个/细胞，黏附能力最强。ttttttttttttt结论ttttt从散养茶花鸡粪便中分离得到唾液乳杆菌、加氏乳杆菌、罗伊氏乳杆菌和约氏乳杆菌4株乳酸菌，其中加氏乳杆菌CHF6-2具有显著胃肠道耐受性、抑菌活性、抗生素敏感性和肠上皮细胞黏附能力等益生特性，可作为微生态制剂候选菌株进一步开展深层次研究。tttt

In vitro testing of commercial and potential probiotic lactic acid bacteria

tttttPurposetttttIn this study, we investigated the probiotic properties of lactic acid bacteria derived from national protected species of the genetic resources of livestock and poultry, Chahua chickens, in order to provide solid base to the potential microbial agent applied in poultry production.tttttttttttttMethodtttttFecal samples of free range Chahua chickens were collected and MRS medium was used to isolate suspected lactic acid bacteria. The strains were subjected to physiological and biochemical tests and 16S rDNA sequence analysis. The probiotic properties of these isolates including resistance to gastrointestinal conditions, antimicrobial activity, susceptibility to antibiotics, and adhesive ability to intestinal epithelial cells were determined.tttttttttttttResultstttttFour strains including CHF4-1, CHF6-2, CHF7-2 and CHF10-2, were isolated. According to the physiological and biochemical identification and 16S rDNA sequence analysis, CHF6-2, CHF10-2, CHF7-2 and CHF4-1 were identified as L. gasseri, L. johnsonii, L. reuteri, and L. salivarius, respectively. Compared with the reference strain L. rhamnosus GG, both L. gasseri CHF6-2 and L. johnsonii CHF10-2 showed greater gastrointestinal tolerance and higher antimicrobial activity. Except being intermediately susceptible to cefotaxime,L. gasseri CHF6-2 was susceptible to the other tested antibiotics. Adhesion ability ofL. gasseriCHF6-2 was the best among these LAB strains with the index of 13.07 ± 0.22.ttttttttttttttttttConclusiontttttFour LAB strains were isolated from fecal sample of Chahua chickens and L. gasseri CHF6-2 showed the best probiotic properties including good gastrointestinal tolerance, higher antimicrobial activity, antibiotic susceptibility and effective adhesion to intestinal epithelial cells. L. gasseri CHF6-2 has the potential to be a probiotic candidate and further study is needed to determine its application in poultry production.ttttttttt