百合品种改良与生物技术研究进展

ttttt目的ttttt探索野生百合在改良百合品种中的潜在价值。ttttttttttttt方法ttttt以百合野生种为父本，栽培品种为母本，通过切割花柱、胚拯救等技术，对具有优良性状野生百合与主要栽培百合远缘杂交的亲和性进行研究。ttttttttttttt结果ttttt6个野生百合种(卷丹、玫红百合、湖北百合、兰州百合、南川百合、大理百合)分别与5个主栽品种(西伯利亚、索邦、西诺红、木门、罗宾娜)远缘杂交组合的子房平均膨大率为68.4%、64.7%、56.5%、80.9%、39.6%、62.7%，表现出较高的子房膨大率，并获得了7个远缘杂交后代。ttttttttttttt结论ttttt以南川百合和卷丹为亲本的杂交组合比其他野生百合更容易获得有胚种子，具有清香味的玫红百合与东方百合栽培品种具有良好的亲和性；供试的6种野生百合与百合主栽品种杂交都有较高的子房膨大率，但真正有胚种子很少，说明子房膨大并不代表胚的发育，仅用子房膨大率评价远缘杂交亲和性并不客观，应把子房膨大率、幼胚萌发率、杂种成活率结合起来综合考虑。tttt

Application of in vitro pollination,ovary culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses

tttttPurposetttttIn order to explore the potential value of wild lily for improving lily cultivars.tttttttttttttMethodtttttFive main cultivars and six wild species with excellent traits were used as experimental materials to study the compatibility of interspecific hybridization, which was done by cutting style, embryo rescue.tttttttttttttResulttttttThe average rate of ovary swelling was 68.4% 64.7%, 56.5%, 80.9%, 39.6%, 62.7% respectively and the hybrids were obtained from 7 crossing combinations when each wild lily - Lilium lancifoluim, L. amoenum, L. henryi, L. davidi var. unicdor, L. rosthornii, L. taliense as the male parent crossed with 5 popular cultivars - Siberia, Sorbonne, Viviana, ConcaD’or, Robina as the female parent.ttttttttttttttttttConclusiontttttThe crossing combinations of L. rosthornii and L. lancifoluim were easier to get embryo seed than others. L. amoenum with scent had a good compatibility with oriental lily cultivars. All crossings of wild lilies and cultivars had a high ovary swelling rate, but the embryo development was very poor, which suggested ovary swelling didn’t represent seed-setting. So ovary swelling rate is not the only index to evaluate the compatibility of distant hybridization. The ovary swelling rate, embryo germination rate and hybrids’ survival rate should be integrated into the evaluation of distant hybridization compatibility.ttttttttt