| 小麦春化相关特异性PCR扩增体系的建立 |
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| 引用本文： | 董爱香,任江萍.小麦春化相关特异性PCR扩增体系的建立[J].山西农业大学学报(自然科学版),2000,20(3):205-207. |
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| 作者姓名： | 董爱香 任江萍 |
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| 作者单位： | 山西农业大学农学院,山西太谷 |
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| 摘 要： | 以小麦幼苗DNA为模板 ,采用小麦春化相关特性Verc2 0 3序列设计合成的春化特异引物 ,进行PCR扩增。通过对扩增体系中引物浓度、Taq酶浓度、Mg2 + 、退火温度以及扩增程序的选择优化 ,确定了PCR扩增的最佳条件 ,获得稳定而理想的扩增效果
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| 关 键 词： | 小麦幼苗DNA 春化特异序列 PCR扩增体系 |
The Bultment of PCR Anplification system of Vernalizing-Related Specitication in Whert |
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| DONG Ai-xing,REN Jiang-pin, YIN Jun.The Bultment of PCR Anplification system of Vernalizing-Related Specitication in Whert[J].Journal of Shanxi Agricultural University,2000,20(3):205-207. |
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| Authors: | DONG Ai-xing REN Jiang-pin YIN Jun |
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| Abstract: | Using specific primer which was synthesized by wheat vemalizing-related specific Verc203sequence,PCRamplification was carried out with DNA of wheat seeding as template.By selection and improving of the concentration of primer Taq DNA polymerase ,Mg 2 ,annealing tempreature and amplifying sequence,the best condition had been decided and the stable,idealistic ampififcation effect had been achieved |
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| Keywords: | wheat seeding DNA vernalizing specific sequence PCRamplification system |
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