Development of a label-free immunosensor system for detecting oocyte maturation-inducing hormone in fish |
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Authors: | Hideaki Endo Tadayoshi Muramatsu Goro Yoshizaki Huifeng Ren Hitoshi Ohnuki |
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Institution: | (1) Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan;(2) Faculty of Marine Technology, Tokyo University of Marine Science and Technology, 2-1-6 Etchujima, Koto-ku, Tokyo 135-8533, Japan |
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Abstract: | A label-free immunosensor for detecting the oocyte maturation-inducing hormone 17, 20β-dihydroxy-4-pregnen-3-one (DHP), was
developed. A principle of the sensor system was based on differences in electrochemical activity changed by an immunoreaction
in the absence and presence of DHP. For preparation of the immunosensor, anti-DHP IgG was immobilized on an Au working electrode
modified with a self-assembled monolayer of 3-mercaptopropionic acid. The sensor was immersed into a sample solution containing
DHP. DHP was determined by cyclic voltammetry. The immunosensor showed a specific response to DHP, and the oxidation peak
current linearly decreased in the range of 7.8–500 pg ml−1 with a correlation coefficient of 0.996. The sensor system was applied to determine the DHP levels in plasma of goldfish
and was compared with the DHP levels of the same samples determined using an ELISA as the conventional method. Good correlation
was obtained between values determined using both methods in the range of 0.1–7.7 ng ml−1 (correlation coefficient 0.876). These findings suggest that the proposed label-free immunosensor can be used to analyze
DHP levels in fish plasma samples. |
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