防风嫩茎无性系建立的研究

为保护野生资源,实现人工栽培,以防风的嫩茎为材料,进行了愈伤组织的诱导和分化、试管苗生根、移栽和定植的研究,成功的建立起防风的无性系。结果证明:MS+BA 0.2 mg/L+2,4-D 2.0 mg/L+NAA 0.5mg/L是愈伤组织诱导培养和增殖继代培养的理想培养基;MS+AgNO30.5 mg/L+6-BA 1.0 mg/L+NAA 0.1mg/L是愈伤组织分化培养的理想培养基;1/3MS+NAA 0.1 mg/L+IAA 0.3 mg/L是不定芽生根培养和试管苗生根继代增值的理想培养基;试管苗易移栽、定植成活;定植的试管苗保持了防风所有植物学性状。

The Study on Clone Construction of Tender Stems of Lythrum salicaria

In order to preserve the wild resources and meet the needs of false planting,tender stems of Lythrum salicaria were used as material to induce callus and differente adventitious buds,rooting,transplantationand stable planting of tube seedlings,then the clone of Lythrum salicaria was successfully established.The results showed that MS+BA 0.2 mg/L+2,4-D 2.0 mg/L+NAA 0.5 mg/L was the perfect medium for callus inducing and callus multiplication;the medium MS+AgNO3 0.5 mg/L +6-BA 1.0 mg/L+NAA 0.1 mg/L was suitable for callus differentiation;the best medium for rooting of adventitious buds and rooting multiplication of tube seedlings was 1/3MS+NAA 0.1 mg/L+ IAA 0.3 mg/L;the transplanted and stable planted seedlings survived easily;The seedlings stable planted maintained all botany characteristics of the Lythrum salicaria.