批量筛选水稻T-DNA插入突变体库获得生殖发育相关突变体

针对花器官形态和种子发育突变表型对大型水稻T-DNA插入突变体库进行筛选,获得了大量突变体信息及材料,在9 760个突变体家系中筛选得到177个花器官形态和数量异常的突变家系,突变频率为1.81%;对9 760个家系中的3 432个家系筛选得到179个种子发育缺陷的突变家系,突变频率为5.22%。对所获得的270个突变家系进行了T-DNA插入的阳性检测,阳性率为64.8%。利用公共数据库RMD(Rice MutantDatabase,RMD)给定的侧翼序列,鉴定了其中1个结实率较低的突变体家系,表明其突变表型和T-DNA插入共分离,为深入研究该基因的功能提供了重要的遗传材料。

Rice mutants of reproductive development obtained from screening the rice mutant library of T-DNA insertional mutagenesis

To identify rice mutants of floral organ and seed development,a rice mutant library of T-DNA insertional mutagenesis constructed by the national key laboratory of crop genetic improvement at Huazhong Agricultural University was screened on large scale.A large number of useful information of mutants were got.Among 9 760 stable lines of phenotype variant,177 lines showed morphology and number change of floral organ,with mutation frequency of 1.81%;179 lines from the 3 432 lines showed abnormal seed development,the percent of mutants was 5.22%.270 lines of phenotype variant interested were obtained and 175 lines showed T-DNA insertion through PCR test,the positive rate was 64.8%,which will lay the foundation for cloning genes related with rice floral organ and seed development.One low seed-setting mutant was identified and confirmed to be cosegregated with its corresponding T-DNA flanking sequences tags,providing an important genetic resource for further studying the function of this gene.