Cloning of the phloem-specific small heat-shock protein from leaves of rice plants |
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| Authors: | Akari Fukuda Yoshihisa Okada Nobuo Suzui Toru Fujiwara Tadakatsu Yoneyama Hiroaki Hayashi |
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| Institution: | 1. Department of Paddy Farming, National Agricultural Research Center for Tohoku Region , Omagari, 014-0102, Japan akfukuda@affrc.go.jp;3. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Bunkyo-ku, Tokyo, 113-8657, Japan;4. Takasaki Radiation Chemistry Research Establishment , Japan Atomic Energy Research Institute , Takasaki, 370-1207, Japan;5. Biotechnology Research Center , The University of Tokyo , Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan;6. PRESTO, JST , Japan |
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| Abstract: | The N-terminal amino-acid sequence of a major rice phloem-sap protein, designated as RPP23, was determined. The complete amino-acid sequence of RPP23 was deduced from the corresponding rice EST- clone and contained an extra 46 amino acids at the N-terminus, that was apparently cleaved off to form mature RPP23 in sieve tubes. RPP23 shared a similarity to plant small heat-shock proteins (smHSPs), though the N-terminal region of RPP23 was distinct from that of known smHSPs. Immunocytological analyses using leaf sections showed that RPP23 was located only in the phloem regions of leaves, and was present in non-stressed plants. In mature leaves, stronger immunocytological signals were detected in sieve elements than in companion cells. |
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| Keywords: | Oryza sativa phloem rice sieve tube small heat-shock protein |
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