A rapid and easy method of detecting levels of gene expression in intact tobacco plants using an Agrobacterium-mediated transient assay: Toward development of nutritional diagnosis based on gene expression |
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| Authors: | Naoko Ohkama Hiroaki Hayashi Tom Fujiwara |
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| Affiliation: | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Bunkyo-ku , Tokyo , 113-8657 , Japan |
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| Abstract: | For the use of gene expression for nutritional diagnosis, a rapid and easy method of detecting levels of gene expression was proposed. Agrobacterium tumefaciens carrying the GUS reporter gene was injected into intact tobacco leaves with a plastic syringe, and transient GUS expression was determined. GUS activities were reproducible in young leaves. |
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| Keywords: | Agrobacterium intact tobacco plants nutritional diagnosis transient gene expression |
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