| 青霉素影响甜菜夜蛾幼虫耐受几种苏云金杆菌晶体蛋白的能力 |
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| 引用本文： | 陈瑞瑞,崔金杰,李国清.青霉素影响甜菜夜蛾幼虫耐受几种苏云金杆菌晶体蛋白的能力[J].植物保护,2011,37(6):112-114. |
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| 作者姓名： | 陈瑞瑞 崔金杰 李国清 |
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| 作者单位： | 1. 南京农业大学植物保护学院/教育部病虫害综合治理重点开放实验室,南京,210095
2. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,安阳,455000 |
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| 基金项目： | 转基因生物新品种培育科技重大专项 |
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| 摘 要： | 本文评估了Cry1Ab、Cry1Ba和Cry1Ca蛋白对甜菜夜蛾2龄幼虫的毒力，其LC50分别为0.442 5、0.675 7 μg/cm2和0.150 2 μg/cm2，Cry1Ba毒力最低，其次是Cry1Ab，而Cry1Ca毒力最高。青霉素影响幼虫对晶体毒素的耐受能力；一次添加高剂量(500 μg/cm2)青霉素于饲料中显著提高幼虫的耐受能力，以含低剂量(60 μg/cm2)青霉素的饲料饲养幼虫多代也能达到相似的效果。
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| 关 键 词： | 苏云金杆菌 晶体毒素 甜菜夜蛾 青霉素 毒力 |
Penicillin ingestion influences toxic effects of several Cry toxins from Bacillus thuringiensis on Spodoptera exigua larvae |
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| Chen Ruirui,Cui Jinjie,Li Guoqing.Penicillin ingestion influences toxic effects of several Cry toxins from Bacillus thuringiensis on Spodoptera exigua larvae[J].Plant Protection,2011,37(6):112-114. |
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| Authors: | Chen Ruirui Cui Jinjie Li Guoqing |
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| Institution: | 1. Key Laboratory of Integrated Management of Crop Diseases and Pests of Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing210095, China;2. Research Institute of Cotton, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang455000, China |
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| Abstract: | Toxicities of Cry1Ab, Cry1Ba and Cry1Ca from Bacillus thuringiensis to the 2nd instar larvae of Spodoptera exigua were evaluated. The LC50 values were 0.442 5 μg/cm2, 0.675 7μg/cm2 and 0.150 2 μg/cm2, respectively. Cry1Ba was least toxic, followed by Cry1Ab, and Cry1Ca was most toxic. Penicillin affected larval tolerance to the 3 Cry toxins. At the concentration of 500 μg/cm2, penicillin mixed directly with the toxins could significantly increase larval tolerance. At the concentration of 60 μg/cm2, however, penicillin should be first added alone to the diet for the larvae for several generations to obtain similar tolerance. |
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| Keywords: | Bacillus thuringiensis Cry toxin Spodoptera exigua penicillin toxicity |
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