Antioxidant activity of extracts from <Emphasis Type="Italic">Calocedrus formosana</Emphasis> leaf,bark, and heartwood |
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Authors: | Sheng-Yang?Wang Jyh-Horng?Wu Sen-Sung?Cheng Chiu-Ping?Lo Hsing-Ning?Chang Lie-Fen?Shyur Email author" target="_blank">Shang-Tzen?ChangEmail author |
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Institution: | (1) Department of Forestry, National Taiwan University, No. 1 Section 4 Roosevelt Road, Taipei, 106, Taiwan, China;(2) Institute of BioAgricultural Sciences, Academia Sinica, Taipei, 115, Taiwan, China |
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Abstract: | Calocedrus formosana Florin (Cupressaceae) is an endemic tree to Taiwan. To evaluate the antioxidant activity of the plant extracts from heartwood, bark, and leaf of C. formosana, assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion scavenging activities, as well as prevention of DNA strand cleavage were performed in this study. Similar IC50 values against the DPPH radical were found for the heartwood and bark extracts at approximately 23µg/ml. Moreover, the heartwood extract exhibited the highest inhibitory activity against superoxide radicals among the test samples; a 2.3-fold lower value of IC50 for superoxide radical inhibition was found in the heartwood extract relative to that of (+)-catechin. Much less effect on inhibition of DPPH and superoxide radicals was found from the leaf extract of C. formosana. More than 70% of superoxide radicals were inhibited in the presence of 10µg/ml heartwood extract, whereas only 15% inhibition was obtained from the leaf extract. The heartwood extract, at a dose of approximately 0.5mg/ml, apparently completely prevented the X174 supercoiled DNA cleavage induced by ultraviolet photolysis of H2O2, as judged by agarose gel electrophoresis. This report also suggests that the antioxidant activities of the plant extracts of C. formosana are in good correlation with their phenolic contents. |
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Keywords: | Calocedrus formosana Heartwood extract Antioxidant activity Total phenolic content |
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