| 黄瓜根际土壤及根系的AMF菌群多样性研究 |
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| 引用本文： | 任 禛,黄鹤平,陈泽斌,等.黄瓜根际土壤及根系的AMF菌群多样性研究[J].广东农业科学,2015,42(6):105-110. |
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| 作者姓名： | 任 禛 黄鹤平 陈泽斌 等 |
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| 作者单位： | 1. 昆明学院农学院,云南昆明650214;云南省高校都市型现代农业工程研究中心,云南昆明650214
2. 昆明学院农学院,云南昆明,650214 |
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| 基金项目： | 云南省应用基础研究自筹经费项目，昆明学院人才引进项目，云南省高校都市型现代农业工程研究中心科研项目 |
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| 摘 要： | 从昆明学院种植基地随机采集黄瓜根系及根际土壤样品,采用巢式PCR技术分别扩增菌根及土壤丛枝菌根真菌(AMF)部分18S rRNA基因区域,利用该产物构建AMF克隆文库,并结合ARDRA筛选、DNA测序和系统发育树分析对黄瓜AMF菌群组成进行研究.结果表明:根际土壤检测到7种AMF:摩西球囊霉(Glomus mosseae)、根内球囊霉(G.intraradices)、地表球囊霉(G.versiforme)以及其他4种未知的Glomus属类型,而根系除发现了G.mosseae、G.intraradices和G.versiforme外,仅检测到了其他2种未知Glomus属种类.
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| 关 键 词： | 丛枝菌根真菌 18S rRNA基因 巢式PCR 系统发育树分析 |
Diversity of arbuscular mycorrhizal fungi community
in the rhizosphere soil and roots of cucumber |
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| REN Zhen,HUANG He-ping,CHEN Ze-bin,ZHANG Yong-fu,YANG Da-hai.Diversity of arbuscular mycorrhizal fungi community
in the rhizosphere soil and roots of cucumber[J].Guangdong Agricultural Sciences,2015,42(6):105-110. |
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| Authors: | REN Zhen HUANG He-ping CHEN Ze-bin ZHANG Yong-fu YANG Da-hai |
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| Abstract: | Cucumber roots and rhizosphere soil samples were collected randomly from Kunming College planting
base. The partial 18S rRNA gene of two samples were amplified by Nested-PCR. These DNA fragments were used to
construct AMF clone libraries. AMF community composition were studied by ARDRA screening, DNA sequencing
and phylogenetic tree analysis. The results showed that seven species were obtained in rhizosphere soil. They were
Glomus mosseae, G. intraradices, G. versiforme and other four unknown Glomus types. Except for G. mosseae, G.
intraradices and G. versiforme, only two unknown Glomus species were detected in roots. |
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| Keywords: | arbuscular mycorrhizal fungi 18S rRNA gene Nested-PCR phylogenetic tree analysis |
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