琅琊鸡Mx基因3'端部分序列PCR-RFLP与序列分析

目的]为琅琊鸡分子标记辅助选择、抗病育种提供依据。方法]利用PCR-RFLP技术对琅琊鸡Mx基因3’端片段的HaeIII的酶切多态性进行分析。结果]琅琊鸡群体中存在Mx基因Haem酶切位点的多态性，且均有A、B2个等住基因，基因频率分别为0．562和0．438。Mx基因Hoe III酶切位点的基因型分布在琅琊鸡群体中极显著偏离Hardy-Weinberg平衡状态（P〈0．01）。对多态片段进行克隆测序分析，结果表明，该扩增片段长度大小为357bp，多态片段存在长度为31bp的碱基缺失。结论]在山东省地方鸡种琅琊鸡眠基因3’端序列中发现存在Hoe III-RFLP。

PCR-RFLP and Sequence Analysis of Mx Gene 3' Sequence in Langya Chicken

Objective] The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method] The genetic polymorphism of Hae Ⅲ site of Mx gene 3' sequence in Langya chicken was analyzed by PCR-RFLP. Result] The results showed that Hae Ⅲ site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae Ⅲ site was significantly not in Hardy-Weinberg equilibrium (P<0.01). The polymorphic fragments were cloned and sequenced, the results revealed the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion] It was found that Hae Ⅲ-RFLP exists in Mx gene 3' sequence in Langya chicken breeds of Shandong Province.