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| 5种脑炎人兽共患病病毒多重RT-PCR检测方法的建立 | |
| 引用本文： | 蔡绪禹,康晓平,刘洪,李裕昌,孙恩成,王凌凤,杨涛,刘霓红,步志高,李文京,杨银辉,吴东来.5种脑炎人兽共患病病毒多重RT-PCR检测方法的建立[J].中国预防兽医学报,2011,33(2). |
| 作者姓名： | 蔡绪禹 康晓平 刘洪 李裕昌 孙恩成 王凌凤 杨涛 刘霓红 步志高 李文京 杨银辉 吴东来 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/农业部兽医公共卫生重点开放实验室,黑龙江,哈尔滨,150001 2. 军事医学科学院微生物流行病研究所病原微生物/生物安全国家重点实验室,北京,100071 3. 中国动物疫病预防控制中心,北京,100125 |
| 摘 要： | 为建立同时检测流行性乙型脑炎病毒(JEV)、森林脑炎病毒(TBEV)、东方马脑炎病毒(EEEV)、西方马脑炎病毒(WEEV)和基孔肯雅病毒(CHIKV)5种人兽共患脑炎病病毒的多重RT-PCR方法,本研究根据GenBank登录的相关病毒基因序列设计特异引物,通过优化引物组合及PCR反应条件,建立可同时检测5种病毒的方法,扩增片段长度分别为411 bp(JEV)、945 bp(TBEV)、193 bp(EEEV)、545 bp(WEEV)和769 bp(CHIKV);该方法具有良好的特异性,对病毒核酸最低检测拷贝数分别为7.1×103、3.6×103、2.2×103、5.6×103和5.1×103.该方法具有特异性强、灵敏度高、操作简便等优点,为以上5种人兽共患脑炎病病毒提供快速检测手段. |
| 关 键 词： | 流行性乙型脑炎病毒 森林脑炎病毒 东方马脑炎病毒 西方马脑炎病毒 基孔肯雅病毒 多重RT-PCR |
Establishment of multiplex PCR for the detection of five encephalitis zoonosis viruses |
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| CAI Xu-yu,KANG Xiao-ping,LIU Hong,LI Yu-chang,SUN En-cheng,WANG Ling-feng,YANG Tao,LIU Ni-hong,BU Zhi-gao,LI Wen-jing,YANG Yin-hui,WU Dong-lai.Establishment of multiplex PCR for the detection of five encephalitis zoonosis viruses[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(2). | |
| Authors: | CAI Xu-yu KANG Xiao-ping LIU Hong LI Yu-chang SUN En-cheng WANG Ling-feng YANG Tao LIU Ni-hong BU Zhi-gao LI Wen-jing YANG Yin-hui WU Dong-lai |
| Institution: | CAI Xu-yu1,KANG Xiao-ping2,LIU Hong2,LI Yu-chang2,SUN En-cheng1,WANG Ling-feng1,YANG Tao1,LIU Ni-hong1,BU Zhi-gao1,LI Wen-jing3,YANG Yin-hui2*,WU Dong-lai1*(1.The Key Laboratory of Veterinary Public Health,Ministry of Agricultural,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China,2.State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,... |
| Abstract: | The serious encephalitis symptoms both in human and in animals could be induced by one of the five encephalitic zoonosis viruses,including Japanese B encephalitis virus(JEV),tick-borne encephalitis virus(TBEV),eastern equine encephalitis virus(EEEV),western equine encephalitis virus(WEEV) and Chikungunya virus(CHIKV).A multiplex PCR method was developed for detecting the 5 virus with a sets of specific primers designed according to the published viral genome sequences.The PCR products for each virus was 411... |
| Keywords: | Japanese B encephalitis virus tick-borne encephalitis virus eastern equine encephalitis virus western equine encephalitis virus Chikungunya virus multiplex RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |