桑天牛长尾啮小蜂对桑枝挥发物的触角电位和嗅觉反应

桑天牛长尾啮小蜂(Aprostocetus fukutai)是蛀干害虫桑天牛(Apriona germari)的重要卵寄生蜂之一,桑天牛危害的桑枝挥发物在该寄生蜂寄主生境定向搜寻过程中起着至关重要的作用。采用触角电位(EAG)仪测定了桑天牛长尾啮小蜂对8种桑枝挥发物的电生理反应,发现该寄生蜂对这8种桑枝挥发物都能产生电生理反应,且随着化合物浓度的增加电生理反应增强。利用Y型嗅觉测定仪测定了桑天牛长尾啮小蜂对不同浓度挥发物的趋性行为,结果显示0.01%月桂烯、0.01%里那醇和1%Z-乙酸-3-己烯酯对该寄生蜂均具有显著的引诱作用。研究结果对揭示桑天牛长尾啮小蜂寄主定向的化学本质具有重要意义。

Electroantennographic and Olfactory Responses of Aprostocetus fukutai to the Volatiles From Mulberry Branch

Aprostocetus fukutai is one of the important egg parasitoids of Apriona germari,the stem borer of many species of trees.The volatiles from Mulberry branch infested by A.germari play the key role in the host habitat location process of the wasp.In this paper,the electrophysiological responses of A.fukutai to 8 volatile chemicals at different concentrations from Mulberry branch were measured by electroantennography(EAG).The results showed that these chemicals all elicited electrophysiological responses of the wasp,which became stronger as the volatile concentration increasing.The taxis behavior of the wasp to the volatiles at different concentrations was also assayed by Y-tube olfactometer.Either myrcene or linalool at the concentration of 0.01% or Z-hexenyl acetate at 1% attracted the wasp significantly,which plays the important role in revealing the chemical nature of host habitat location for A.fukutai.