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树仔菜植株多糖的提取与测定
引用本文:陈丽霞,韩丙军,周楷博,姜欣,彭黎旭.树仔菜植株多糖的提取与测定[J].热带农业科学,2010,30(11):30-32.
作者姓名:陈丽霞  韩丙军  周楷博  姜欣  彭黎旭
作者单位:[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室,海南海口571101 [2]琼台师范高等专科学校数理系,海南海口571100 [3]中国热带农业科学院分析测试中心,海南海口571101
基金项目:中央级公益性科研院所基本科研业务费专项,海南省自然科学基金,海南省高等学校科学研究项目
摘    要:采用超声萃取法、浸泡提取法和加热回流提取法进行树仔菜多糖的提取研究,并用苯酚-硫酸法进行多糖含量测定。结果表明,超声萃取法提取效率最高,树仔菜植株中多糖含量为0.36%。该分析方法简便、快速、准确、灵敏度好。

关 键 词:树仔菜  多糖  提取  测定

Extraction and Determination of Polysaccharide in Sauropus androgynus (z) Meer
CHEN Lixia,HAN Bingjun,ZHOU Kaibo,JIANG Xin,PENG Lixu.Extraction and Determination of Polysaccharide in Sauropus androgynus (z) Meer[J].Chinese Journal of Tropical Agriculture,2010,30(11):30-32.
Authors:CHEN Lixia  HAN Bingjun  ZHOU Kaibo  JIANG Xin  PENG Lixu
Institution:1 Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology/ Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101, China; 2 Mathematical Department, Qiongtai Teachers College, Haikou, Hainan 571100, China; 3. Analysis and Testing Center, CATAS, Haikou, Hainan 571101, China)
Abstract:The methods of extraction of polysaccharide from Sauropus androgynus(z) Meer were studied,and determination of the polysaccharide was conducted with the phenol-sulfuric acid method.The results showed that ultrasonic-assisted extraction of the polysaccharide in Sauropus androgynus(z) Meer was most efficient.The content of polysaccharide in Sauropus androgynus(z) Meer was about 0.362%.The method was simple,rapid,and accurate.
Keywords:Sauropus androgynus(z) Meer  polysaccharide  extraction  determination
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