陆地棉抗黄萎病性状的遗传及分子标记研究

 以陆地棉抗黄萎病品系常96和感病品种军棉1号为研究材料，构建了一个含有138个F₂单株的作图群体。利用强致病力菌株VD8对P₁、P₂、F₁、F_2：3群体进行营养钵接种，估算各世代的相对病指。应用主基因 多基因混合遗传模型分析得出该组合的最适遗传模型为C-0模型，即加性-显性-上位性多基因模型。对1998对SSR引物和230对SRAP引物进行筛选，获得148个SSR和6个SRAP多态性标记位点，进一步利用MAPMAKER作图软件，构建了一张含122个标记位点的陆陆杂种遗传连锁图。利用复合区间作图法在第9染色体NAU462-JESPR114区间内，检测到1个抗黄萎病QTL，可解释的表型变异为13.8%。

Inheritance and Molecular Tagging of Resistance against Verticillium Wilt in Upland Cotton

Molecular marker-assisted selection was effective to quickly breeding cultivars resistant to Verticillium wilt. In this research, the upland cotton strain Chang 96, which is resistant against Verticillium wilt, and the susceptible variety, Junmian 1, were used as experimental materials, a tagging population with 138 F₂ individual plants was developed. By artificially inoculating the strongly pathogenic fungi strain to the populations P₁, P₂, F₁, F_2:3, the relative disease indexes of each generation was estimated. Using the method of the major gene plus polygene mixed genetic model analysis between major gene and multiple genes, the optimum genetic model of that combination was discovered to be C₀ model, i.e., the multiple gene model among additive-dominant-epistatic genes. A total of 1998 pairs of SSR primers, and 230 pairs of SRAP primers were screened, 148 SSR and 6 SRAP polymorphism marker loci were obtained. Furthermore, by utilizing Mapmaker software, a genetic linkage map based on intraspecific F₂ populations with 122 marker loci was constructed. According to the composite interval principle to scan total cotton genome by Mapmaker/QTL program, one resistance QTL was detected and localized on chromosome nine between the interval of the NAU462 and JESPR114, 13.8% phenotypic variance in F_2:3 of which might be explained.