侵染假酸浆的泰国番茄黄化曲叶病毒全基因组结构特征

为明确假酸浆Nicandra physalodes叶片黄化、皱缩症状是否由菜豆金色花叶病毒属病毒侵染引起，本研究利用分子检测方法和生物信息学技术鉴定了假酸浆样品中的病毒种类。从采集的病样中克隆并获得了2条菜豆金色花叶病毒属病毒DNA-A全序列和1条beta卫星全序列，经全序列分析发现，该双生病毒的两条DNA-A全序列与泰国番茄黄化曲叶病毒(tomato yellow leaf curl Thailand virus, TYLCTHV)云南分离物TYLCTHV-YN1732一致性最高，达99.3%,亲缘关系较近；beta卫星的全序列与云南番茄曲叶beta卫星(tomato leaf curl Yunnan betasatellite, TLCYnB)的分离物YN5230一致性最高，达99.3%,亲缘关系较近。重组分析显示，假酸浆上分离的TYLCTHV-YN5735-12是一个重组病毒，有两个重组事件，一个主要发生在AV1的编码区，由中国番茄黄化曲叶病毒(tomato yellow leaf curl China virus, TYLCCNV)和广西大戟曲叶病毒(euphorbia lea...

Characterization of the genome structure of tomato yellow leaf curl Thailand virus infecting Nicandra physalodes

Molecular detection and bioinformatics analysis were performed to determine whether Nicandra physalodes plants in Yunnan province displaying yellow and crumple symptoms were associated with begomovirus infections. Two complete begomovirus DNA-A molecules and one betasatellite molecule were obtained from infected samples. Sequence comparison revealed that the two DNA-A sequences shared the highest identity (99.3%) with the tomato yellow leaf curl Thailand virus (TYLCTHV) isolate TYLCTHV-YN1732, while the betasatellite shared the highest sequence identity (99.3%) with the tomato leaf curl Yunnan betasatellite (TLCYnB) isolate YN5230. Recombination analysis revealed that the begomovirus infecting Nicandra physalodes was a recombinant virus derived from two potential recombinant events. One recombination event was detected in the coding region of AV1, contributed by tomato yellow leaf curl China virus (TYLCCNV) and euphorbia leaf curl Guangxi virus (EuLCGXV). The other recombination event occurred mainly in the intergenic region (IR), with the two parents being tobacco leaf curl Thailand virus (TbLCTHV) and TYLCTHV. This study recorded Nicandra physalodes as a new host for TYLCTHV. This is the first report of a monopartite begomovirus in association with a betasatellite infecting Nicandra physalodes.