Colonization,proliferation, and survival of intraperitoneally transplanted yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis> spermatogonia in nibe croaker <Emphasis Type="Italic">Nibea mitsukurii</Emphasis> recipient |
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Authors: | Kentaro Higuchi Yutaka Takeuchi Misako Miwa Yoji Yamamoto Kazunobu Tsunemoto Goro Yoshizaki |
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Institution: | (1) Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan;(2) Research Center for Advanced Science and Technology, Tokyo University of Marine Science and Technology, Banda 670, Tateyama Chiba, 294-0308, Japan;(3) Present address: Amami Station, National Center for Stock Enhancement, Fisheries Research Agency, Hyou, Setouchi Kagoshima, 894-2414, Japan; |
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Abstract: | Recently, we developed an intraspecies spermatogonial transplantation technique in a pelagic egg spawning marine teleost,
nibe croaker Nibea mitsukurii. Nibe croaker is an ideal candidate recipient for spermatogonial transplantation since it has a short generation time and
small body size. In the present study, yellowtail Seriola quinqueradiata spermatogonia were transplanted into nibe croaker larvae, and the behavior of transplanted spermatogonia in recipient gonads
was observed. Three weeks post-transplantation, yellowtail spermatogonia were incorporated into the gonads of 72 out of 88
recipients. An antiproliferating cell nuclear antigen was detected in incorporated yellowtail spermatogonia, suggesting that
the xenogenic germ cells were proliferating in recipient gonads. Yellowtail vasa-positive spermatogonia survived for 11 months after transplantation in the gonads of recipient fish. Thus, we showed that
the microenvironment in nibe croaker gonads can support the colonization, proliferation, and survival of germ cells derived
from a different taxonomic family. |
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