3个山羊品种KAP6.2和KAP7基因的PCR-SSCP分析

采用PCR-SSCP法对３个山羊品种角蛋白辅助蛋白（KAP6.2，KAP7）进行了多态性研究。结果显示，３个品种中KAP7均未出现多态，而KAP6.2均出现了３种带型：AA、BB和AB。Hardy-Weinberg分析表明辽 苛高代杂种山羊和黎城大青羊未达到平衡状态，辽宁绒山羊达到了平衡。测序结果显示，和GenBank登陆序列相比，AA和BB均发生了碱基变化。其中AA型出现了以下变异：107 bp(A-G)，196 bp(C-T)，199 bp(T-G)，252 bp(A-T)，265 bp(C-T)，291 bp(A-G)，292 bp(T-C)，345 bp(T-G);BB型出现了以下变异：164 bp(T-C)，194 bp(T-C)，199 bp(T-G)，201 bp(C-G)，233 bp(A-G)，275 bp(A-G)，299 bp(A-G)，309 bp(G-C)，327 bp(A-G)，352 bp(G-T)。分析表明AA型和BB型变异均造成了氨基酸序列的变化。

Analysis of Polymorphism on KAP6.2 and KAP7 Gene in Three Goat Breeds with PCR-SSCP

The polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP）was used to detected the polymorphism of keratin associated protein(KAP6.2, KAP7) in three goat breeds. The results show that the KAP7 didn''t present polymorphism in all three breeds while the KAP6.2 shows three Genotypes: AA, AB and BB. The frequency of all pattern types analyzed by PCR-SSCP were Hardy We inberg equilibrium in Liaoning goat breed, however the same result didn''t appear in Liaoning-Kelan crossed goat breed and LiCheng goat breed. The different genotype homozygote on KAP6.2 were cloned and sequenced. The results indicated that both AA and BB have nucleotide substitution , the AA genotype presents the following mutation: 107 bp(A-G)，196 bp(C-T)，199 bp(T-G)，252 bp(A-T)，265 bp(C-T)，291 bp(A-G)，292 bp(T-C)，345 bp(T-G)，and the BB genotype: 164 bp(T-C)，194 bp(T-C)，199 bp(T-G)，201 bp(C-G)，233 bp(A-G)，275 bp(A-G)，299 bp(A-G)，309 bp(G-C)，327 bp(A-G)，352 bp(G-T)。The sequence of amino acids was analyzed, the result showed that some of the mutations in AA and BB genotype caused amino acid change.