| 马来西亚甜龙竹的组织培养繁殖试验 |
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| 引用本文： | 龚力波,郑思乡,肖龙骞,李枝林. 马来西亚甜龙竹的组织培养繁殖试验[J]. 西部林业科学, 2003, 0(1): 5-7 |
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| 作者姓名： | 龚力波 郑思乡 肖龙骞 李枝林 |
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| 作者单位： | 1. 云南省科技厅农村服务中心,云南,昆明,650051
2. 云南农业大学,云南,昆明,650201 |
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| 摘 要： | 以马来西亚甜龙竹的当年生枝条作为外植体 ,用MS、 1/2MS、 1/3MS、 1/4MS作为基本培养基 ,并添加不同种类和浓度的植物激素 ,进行组织培养繁殖试验。结果筛选出了各培养阶段最适宜的培养基配方 ,它们分别是 :(1)初代培养基配方 :MS +6 -BA 1 0 0mg/L +0 0 1%PVP +0 1%Vc (2 )丛生芽继代增殖培养基配方 :MS +6 -BA 2 0 0mg/L +KT 1 5 0mg/L +IBA 1 2 5mg/L (3)诱导生根培养基配方 :1/2MS +NAA 1 0 0mg/L +IBA 0 5 0mg/L +PP3 3 3 0 10mg/L。
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| 关 键 词： | 马来西亚甜龙竹 组织培养 繁殖试验 |
| 文章编号： | 1007-3353(2003)01-0005-03 |
| 修稿时间： | 2002-04-04 |
Tissue Culture Experiment of Dendrocalamus asper |
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| GONG Li bo ,ZHENG Si xiang ,XIAO Long qian ,LI Zhi lin. Tissue Culture Experiment of Dendrocalamus asper[J]. Journal of West China Forestry Science, 2003, 0(1): 5-7 |
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| Authors: | GONG Li bo ZHENG Si xiang XIAO Long qian LI Zhi lin |
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| Affiliation: | GONG Li bo 1,ZHENG Si xiang 2,XIAO Long qian 2,LI Zhi lin 2 |
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| Abstract: | Using the annual shoot as the explant, MS, 1/2 MS, 1/3 MS and 1/4 MS as the minimal medium, and adding phytohormone of various concentrations, the propagation experiment of Dendrocalamus asper was conduced. The favorable medium preparations for different culture phases were screened out. They are: 1) Primary culture: MS + 6-BA 1 00 mg/L + 0 01 % PVP + 0 1 % Vc; 2) Secondary culture of caespitose bud: MS + 6-BA 2 00 mg/L + KT 1 50 mg/L + IBA 1 25 mg/L; 3) Rooting induction: 1/2 MS + NAA 1 00 mg/L + IBA 0 50 mg/L + PP 333 0 10 mg/L. |
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| Keywords: | Dendrocalamus asper tissue culture propagation experiment |
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