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电解水对金黄色葡萄球菌生物被膜的影响
引用本文:石文琪,刘建辉,胡树样,李可心,李春立,孙纪录. 电解水对金黄色葡萄球菌生物被膜的影响[J]. 河北农业大学学报, 2017, 40(4). DOI: 10.13320/j.cnki.jauh.2017.0085
作者姓名:石文琪  刘建辉  胡树样  李可心  李春立  孙纪录
作者单位:1. 河北农业大学食品科技学院,河北保定,071001;2. 河北农业大学理工学院,河北黄骅,061100;3. 中国科学院微生物研究所,北京,100101
摘    要:为了阐明不同性质的电解水在控制金黄色葡萄球菌生物被膜中的作用,本研究分别使用酸性、碱性和中性电解水处理生物被膜,并测定处理前后生物被膜生物量、活性、活菌数和构造的变化。结果表明,酸性电解水能够有效杀死生物被膜中的菌体,但不能有效去除生物被膜;其有效氯含量与杀菌作用呈正相关。碱性电解水能够有效去除生物被膜,其pH值直接影响去除能力。中性电解水的作用与酸性电解水相似。不锈钢表面的生物被膜用碱性、酸性和中性电解水处理10min后,活菌数从7.5lgCFU/cm2分别减少至5.3,2.3和3.2lgCFU/cm2。电解水有潜力成为生物被膜杀菌剂和去除剂。

关 键 词:金黄色葡萄球菌  生物被膜  电解水  去除  杀灭

Influence of electrolyzed water on Staphylococcus aureus biofilm
SHI Wen-qi,LIU Jian-hui,HU Shu-yang,LI Ke-xin,LI Chun-li,SUN Ji-lu. Influence of electrolyzed water on Staphylococcus aureus biofilm[J]. Journal of Agricultural University of Hebei, 2017, 40(4). DOI: 10.13320/j.cnki.jauh.2017.0085
Authors:SHI Wen-qi  LIU Jian-hui  HU Shu-yang  LI Ke-xin  LI Chun-li  SUN Ji-lu
Abstract:To elucidate the effects of electrolyzed water with different properties in controlling Staphylococcus aureus biofilm,acidic,basic and neutral electrolyzed water(AEW,BEW and NEW) were used to treat biofilms,and the changes of biofilm biomass,biofilm viability,viable count and biofilm structure were determined.The results showed that AEW could efficiently kill biofilm-imbedded cells,but it could not effectively remove biofilm.The available chlorine content in AEW is positively correlated with bactericidal activity.BEW could effectively re move established biofilm,and the pH of BEW affected removal efficacy.The effects of NEW on S.aureus biofilm were similar to those of AEW.After treatment for 10 min with BEW,AEW and NEW,the viable counts in biofilm on the surface of stainless steel coupons were decreased from 7.51 lgCFU/cm2 to 5.3,2.3 and 3.2 lgCFU/cm2,respectively.AEW,BEW and NEW could be applied as a bactericide and removing agent for S.aureus in biofilm.
Keywords:Staphyloccocus aureus  biofilm  electrolyzed water  removal  killing
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