杂种落叶松连续繁殖与插穗生根关系的生理研究

观察了日×长杂种落叶松连续繁殖插穗不定根发育进程,研究了连续繁殖对插穗生根力衰退的阻滞效应,并分析了一轮采穗圃与原株采穗圃插穗不定根发育期内源激素含量的动态变化。结果表明:扦插后13 31 d是愈伤组织形成和不定根原始体分化发育的关键期,此时一轮分生株愈伤组织形成和不定根发育均优于原株。连续繁殖对插穗生根性状有显著作用,尤其是生根率很低的原株,经过一轮繁殖后,生根性状有极显著提高。连续繁殖影响插穗自身激素含量,尤其是IAA,经过一轮繁殖后,含量明显高于原株。从不定根发育过程中激素的动态变化看,一轮分生株插穗(IAA+GA3+ZR)/ABA比值在不定根发育前期明显高于原株,与生根率的变化一致,可用来衡量不同繁殖次数插穗生根性状的优劣。

Physiological Research on the Relationship between Repeated Cutting and Rooting of Larix

The process of adventitious root development of original and partial ortet cuttings of Larix was investigated in the experiment.The effect of repeated cutting on cuttings’ rooting was studied,and the changes of endogenous hormones content in different stages of adventitious root development were analyzed.The results showed that 13-31 days after cottage were the period key to rooting,the formation rate of callus and the growth speed of young roots of partial ortet cuttings were higher than that of the original ones.The effect of repeated cutting on cuttings’ rooting was remarkable,especially the low-rooting percentage ortets,the rooting traits were remarkably improved.The repeated cutting impacted on the self-owned hormone contents,especially IAA,which was obviously higher than that in original ortet cuttings.The hormone ratio of(IAA+GA3+ZR)/ABA of partial ortet cuttings was higher than the original ones during adventitious root development,and the trend was similar to the change trend of rooting ability,which could be used as an index of rooting ability between original and partial ortet cuttings.