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| 激素对复叶槭茎段和叶片愈伤组织诱导及再生的影响 | |
| 引用本文： | 张彦妮,董亚茹,卓丽环,张远东.激素对复叶槭茎段和叶片愈伤组织诱导及再生的影响[J].林业科学研究,2012,25(4):516-520. |
| 作者姓名： | 张彦妮 董亚茹 卓丽环 张远东 |
| 作者单位： | 1. 东北林业大学园林学院,哈尔滨,150040 2. 东北林业大学园林学院,哈尔滨 150040;上海农林职业技术学院,上海 201600 3. 中国林业科学研究院森林生态环境与保护研究所,北京,100091 |
| 基金项目： | 中央高校基本科研业务费专项资金项目(DL12CA37) |
| 摘 要： | 以复叶槭的茎段、叶片为外植体,以MS为基本培养基,附加多种激素配比试验,结果表明:叶外植体在各种培养基上均形成愈伤组织,但没有出现芽的分化。茎段外植体在多种培养基上均产生愈伤组织,但仅在MS+0.000 5mg.L-1TDZ+0.01 mg.L-1NAA培养基上出现再生芽,芽的分化率为22.8%,在MS+0.1 mg.L-1NAA上生根率为83.3%,移植后成活率为86.2%。 |
| 关 键 词： | 复叶槭 植物激素 愈伤组织 诱导 |
| 收稿时间： | 2011/2/20 0:00:00 |
Effect of Plant Hormones on Callus Induction and Regeneration of Stem Segment and Leaf from Acer negundo |
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| ZHANG Yan-ni,DONG Ya-ru,ZHUO Li-huan and ZHANG Yuan-dong.Effect of Plant Hormones on Callus Induction and Regeneration of Stem Segment and Leaf from Acer negundo[J].Forest Research,2012,25(4):516-520. | |
| Authors: | ZHANG Yan-ni DONG Ya-ru ZHUO Li-huan and ZHANG Yuan-dong |
| Institution: | College of Landscape Architecture, Northeast Forestry University, Harbin 150040, Heilongjiang, China;College of Landscape Architecture, Northeast Forestry University, Harbin 150040, Heilongjiang, China;College of Landscape Architecture, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Shanghai Vocational and Technical College of Agriculture and Forestry, Shanghai 201600, China;;Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China |
| Abstract: | The stem segments and leaves of Acer negundo as explants were put on MS basis culture media with various hormones matching. The results indicated that the callus was induced from leaves on all kinds of culture media, but without the adventitious buds obtained. The callus induced from stem segments was found on several types of culture media, while the regeneration buds were only found on MS+0.000 5 mg·L-1 TDZ + 0.01 mg·L-1 NAA and differentiation ratio of buds was 22.8%. The rooting rate was 83.3% on MS + 0.1 mg·L-1 NAA. The survival rate of transplantion was 86.2%. |
| Keywords: | Acer negundo plant hormone callus induction |
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