油菜蜂花粉醇提工艺的优化

本实验以未破壁和破壁油菜蜂花粉为材料，通过正交试验获得两种花粉最佳醇提工艺。未破壁和破壁油菜蜂花粉最佳醇提工艺分别为：固液比1:8、提取时间2 h、提取温度55℃、震荡频率145 rpm和固液比1:8、提取时间2 h、提取温度65℃，震荡频率145 rpm。在各自最佳醇提工艺条件下，未破壁和破壁花粉的平均提取率存在极显著差异（P<0.01），分别为32.00％和43.16％。以破壁油菜蜂花粉为原料，可极显著地提高提取率。本文为油菜蜂花粉加工过程中普遍应用的醇提工艺环节，提供优化的工艺实验数据和原料选择的依据。

Technology of the Alcohol Extraction from Rape Pollen

The optimized conditions of the alcohol extraction of the wall-unbroken and wall-broken of rape pollen were obtained through orthogonal design.The results showed that the optimized conditions of the wall-unbroken and wall-broken of rape pollen were as follows：ratio of solid-liquid l：8,time 2 h,temperature 55℃,concussion frequency 145 rpm and ratio of solid-liquid l：8,time 2 h,temperature 65℃,concussion frequency 145 rpm.Under these optimized conditions,the ratios of extraction of the wall-unbroken and wall-broken of rape pollens were significant difference （P0.01）.They were 32.00% and 43.16% respectively.The wall-broken of rape pollen was better for more ratio of extraction.In this paper,the optimized conditions of the alcohol extraction and choice of materials during processing of rape pollen were offered.