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福建漳浦太平洋牡蛎微卫星等位基因比较
引用本文:黄勤,陈曦.福建漳浦太平洋牡蛎微卫星等位基因比较[J].福建水产,2011,33(1):1-8.
作者姓名:黄勤  陈曦
作者单位:福建省农业科学院生物技术研究所,福建,福州,350003
基金项目:《福建省科技厅重点计划项目》
摘    要:本文采用Ucdcg153、157、202微卫星分子标记对福建漳浦从广东饶平引人的太平洋牡蛎育苗亲贝进行等位基因比较.两组样品(Cg♀和cg♂)携带的等位基因在微卫星重复区间及其附近存在高度相似的变异特征.这些特征区间分别是:Ucdcg153 23~40位碱基区间,Ucdcg157 73~100位碱基区间,Ucd-cg2...

关 键 词:太平洋牡蛎Crassostrea  gigas  微卫星  近亲繁殖  等位基因

Sequence variation at microsatellite loci of the Pacific oyster Crassostrea gigas from Zhangpu,Fujian
HUANG Qin,CHEN Xi.Sequence variation at microsatellite loci of the Pacific oyster Crassostrea gigas from Zhangpu,Fujian[J].Journal of Fujian Fisheries,2011,33(1):1-8.
Authors:HUANG Qin  CHEN Xi
Institution:(Biotechnology Institute,Fujian Academy of Agriculture Sciences,Fuzhou 350003,China)
Abstract:Samples of Crassostrea gigas from an aquaculture farm located at Zhangpu,Fujian Province,were analyzed for microsatelite sequence variations at Ucdcg153,157 and 202 loci.The two samples(marked as Cg♀and Cg♂) were shown to have similar sequential variations at,or adjacent to,the repeat areas.They involved DNA bases between 23 and 40 of Ucdcg153,73-100 of Ucdcg157,33-55 and 130-147 of Ucdcg202.DNA sequential similarity between the two isoloci of Ucdcg202 was shown to be 97.3%.Results of this investigation revealed a serious inbreeding problem in local oyster hatchery.It is suggested that genetic consultancy be introduced into local aquaculture management,so as to help the farmers breed better oysters by applying appropriate hybrid techniques.
Keywords:Pacific oyster  Crassostrea gigas  microsatellite  inbreeding  isoloci
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