| PCR用于鸭瘟病毒诊断的研究 |
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| 引用本文： | 马秀丽,宋敏训,李玉峰,李峰,王春玲,黄兵,艾武.PCR用于鸭瘟病毒诊断的研究[J].中国预防兽医学报,2005,27(5):408-411. |
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| 作者姓名： | 马秀丽 宋敏训 李玉峰 李峰 王春玲 黄兵 艾武 |
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| 作者单位： | 山东省农业科学院,家禽研究所禽病研究中心,山东,济南,250023 |
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| 摘 要： | 根据鸭瘟病毒UL6和UL7基因序列,设计合成了一对引物,以2株疫苗株、1株强毒株和1株山东分离株DNA为模板,进行PCR扩增,得到预期690bp的目的片段.将扩增的目的片段克隆到pMD18-T载体,经Amp平板筛选,HindⅢ、BamHⅠ双酶切鉴定,获得阳性重组质粒.对重组质粒进行序列测定,与参考序列比较,山东分离株与参考序列的同源性为99.7%,其余3株DPV与参考序列的同源性均为100%.应用PCR可检测人工感染和自然感染鸭瘟的组织中的鸭瘟病毒,表明PCR检测鸭瘟病毒具有很高的特异性、敏感性,该法能够用于鸭瘟急性及亚临床感染的检测与诊断.
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| 关 键 词： | 鸭瘟病毒 诊断 |
| 文章编号： | 1008-0589(2005)05-0408-04 |
| 收稿时间： | 2004-05-12 |
| 修稿时间： | 2004年5月12日 |
Diagnosis of duck plague virus by polymerase chain reaction |
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| MA Xiu-li,SONG Min-xun,LI Yu-feng,Li Feng,WANG Chun-ling,HUANG Bing,AI Wu.Diagnosis of duck plague virus by polymerase chain reaction[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(5):408-411. |
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| Authors: | MA Xiu-li SONG Min-xun LI Yu-feng Li Feng WANG Chun-ling HUANG Bing AI Wu |
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| Abstract: | According to the UL6 and UL7 gene data of duck plague virus(DPV),a pair of primers were designed and used for a polymerase chain reaction(PCR) with two duck plague vaccine strains and one standard strain and one field isolate.A specific 690bp DNA fragment was amplified,which was cloned into PMD18-T vector,and the positive ricombinant clone was selected by the Amp agar plate and characterized by Hind III and BamHI enzyme digestion.The recombinant plasmids were sequenced and compared with pulished sequence.The homology of Shandong isolated strain and the others was 99.7?% and 100?% respectively.The DPV specific DNA fragment was amplified from the infected organs samples of DPV.The PCR is specific and sensitive method which can be used for detection and diagnosis of latent and subclinical DPV infection. |
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| Keywords: | PCR |
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