| 水分胁迫对玉米幼叶质膜ATPase活性影响及其与钙调素和渗透调节的关系 |
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| 引用本文： | 黄国存,周洪显,崔四平,马春红,魏建昆. 水分胁迫对玉米幼叶质膜ATPase活性影响及其与钙调素和渗透调节的关系[J]. 华北农学报, 1997, 0(1) |
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| 作者姓名： | 黄国存 周洪显 崔四平 马春红 魏建昆 |
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| 作者单位： | 河北省农林科学院农业物理生理生化研究所 |
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| 摘 要： | ０．５ＭＰａＰＥＧ－６０００胁迫２４ｈ明显刺激三叶期玉米幼苗，第三叶质膜ＡＴＰａｓｅ活性提高，并且Ｖｍａｘ增加近一倍，而Ｋｍ值却降低；水分胁迫下渗透调节因子脯氨酸累积，Ｋ含量则呈现先上升后下降的趋势，渗透调节的起动与质膜ＡＴＰａｓｅ活性上升可能无直接关系。实验也表明质膜ＡＴＰａｓｅ活性被三氟拉嗪（ＴＦＰ）促进而受钙调素抑制，由此推断质膜ＡＴＰａｓｅ在钙调素参与的磷酸化后活性降低。
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| 关 键 词： | 玉米 幼叶 质膜 水分胁迫 钙调素 渗透调节 |
Effect of Water Stress on Plasma Membrane ATPase Activity of Maize Leaves and Its Relation to Osmoregulation of CaM |
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| Huang Guocun Zhou Hongxian Cui Siping Ma Chunhong Wei Jiankun. Effect of Water Stress on Plasma Membrane ATPase Activity of Maize Leaves and Its Relation to Osmoregulation of CaM[J]. Acta Agriculturae Boreali-Sinica, 1997, 0(1) |
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| Authors: | Huang Guocun Zhou Hongxian Cui Siping Ma Chunhong Wei Jiankun |
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| Abstract: | The PM ATPase activity of the third maize leaves significantly increased in the three leaves seedlings under 0.5MPa PEG 6000 for 24 hours,and V max rised almost one time,but K m decreased.With the development of water stress,proline accumulated, treatment of vanadate, a inhibitor of ATPase ,delay proline accumulation 24 hours. Total contents rised,then declined with or without vanadate. These suggested that the start of osmoregulation may be not related with the increasing of ATPase activities. Trifluoperazine,a inhibitor of calmodulin, stimulated calmodulin and inhibited PM ATPase activities. It suggested that ATPase activities may be declined after phosphorylation. |
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| Keywords: | Maize leaves Plasma membrane ATPase Water stress Calmodulin Osmoregulation |
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