| 昆明地区香石竹斑驳病毒的鉴定、提纯及抗血清制备 |
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| 引用本文： | 卜云萍,丁骅孙,王启芳.昆明地区香石竹斑驳病毒的鉴定、提纯及抗血清制备[J].植物保护,1998,24(5):5-7. |
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| 作者姓名： | 卜云萍 丁骅孙 王启芳 |
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| 作者单位： | 云南师范大学生命科学系(卜云萍)，云南大学生物系(丁骅孙)，云南省工业微生物发酵工程重点实验室(王启芳) |
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| 摘 要： | 在昆明地区栽培的香石竹上发现了香石竹斑驳病毒，人工接种可局部侵染苋色藜、墙生藜、千日红及番杏，可系统侵染昆诺阿藜和美国石竹，分别造成褪绿斑、斑驳或坏死斑。经二轮ＰＥＧ（分子量６０００）沉淀，病毒提纯量约２２０ｍｇ／ｋｇ鲜组织。电镜观察病毒颗粒直径为２８ｎｍ，等轴对称。提纯物紫外扫描呈典型病毒核蛋白吸收峰，Ａ２６０／Ａ２８０值为１．５６。提纯病毒经４次免疫家兔，所得抗血清经试管沉淀测定效价为１∶４０９６，琼脂糖双扩散效价为１∶１０２４。
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| 关 键 词： | 香石竹斑驳病毒 鉴定 提纯 抗血清制备 |
| 修稿时间： | 1998-01-15 |
Identification and purification of carnation mottle virus in Kunming and its antiserun preparation. |
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| Bu Yunping,Ding Huasun,Wang Qifang.Identification and purification of carnation mottle virus in Kunming and its antiserun preparation.[J].Plant Protection,1998,24(5):5-7. |
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| Authors: | Bu Yunping Ding Huasun Wang Qifang |
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| Institution: | Deparment of Life Science;Yunnan Normal University. Kunming 650092;Department of Biology;Yunnan University. Kunming;Key Laboratory of Fermentive Engineering of Industrial Microbiology. Kunming 650091 |
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| Abstract: | Carnation mottle virus was found from carnation plants grown in Kunming District. It could locally infected Chenopodium amaranticolor, C. murale, Gomphrena globosa, Tetragonia expansa, and systemically infected C. quinoa, Dianthus barbatus, and induced chlorotic lesion, mottle or necrotic spots on these hosts respectively. By two cycles of PEG (MW 6000) precipitation, the virus yield was about 220 mg/kg in fresh tissues. Diameter of the isometric particle was 28 nm. With UV absorption spectrum scaning, the ratio of A260 /A280 was 1.56. The titre of antiserum was 1∶4 096 in convention tube precipitation test and 1∶1 024 in gel double diffusion test. |
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| Keywords: | carnation mottle virus identification PEG precipitation antiserum preparation |
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