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| 甘蔗花叶病毒外壳蛋白基因的克隆及系列表达载体的构建 | |
| 引用本文： | 刘小红,荣廷昭,谭振波.甘蔗花叶病毒外壳蛋白基因的克隆及系列表达载体的构建[J].吉林农业大学学报,2007,29(2):133-138. |
| 作者姓名： | 刘小红 荣廷昭 谭振波 |
| 作者单位： | 1. 西华师范大学生命科学学院,南充,637002;四川农业大学玉米研究所,雅安,625014;北京大北农农业科技研究院,北京,100085 2. 四川农业大学玉米研究所,雅安,625014 3. 北京大北农农业科技研究院,北京,100085 |
| 摘 要： | 采用RT-PCR法,从表现玉米矮花叶病症状的玉米叶片中克隆了外壳蛋白(Coat protein)基因。测序和同源性比较结果表明:所克隆的CP基因来自甘蔗花叶病毒(Sugarcane mosaic virus,SCMV)MDB株系,编码219个氨基酸。以CP基因为目的基因,构建了用于基因枪转化的p35SCPfBar,p35SCPrBar,pUbiCPfBar,pU-biCPrBar 4个表达载体和用于农杆菌转化的pCAMBIACPf,pCMABIACPr 2个表达载体。 |
| 关 键 词： | 玉米矮花叶病 甘蔗花叶病毒 外壳蛋白 表达载体 |
| 文章编号： | 1000-5684（2007）02-0133-06 |
| 修稿时间： | 2006-05-082006-09-12 |
Cloning of CP Gene of SCMV and Construction of Several Expression Vectors |
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| LIU Xiao-hong,RONG Ting-zhao,TAN Zhen-bo.Cloning of CP Gene of SCMV and Construction of Several Expression Vectors[J].Journal of Jilin Agricultural University,2007,29(2):133-138. | |
| Authors: | LIU Xiao-hong RONG Ting-zhao TAN Zhen-bo |
| Abstract: | The SCMV(Sugarcane mosaic virus) CP(Coat protein) gene was cloned from maize leaf with maize dwarf mosaic symptom by RT-PCR method.Sequencing showed that the isolated gene was derived from SCMV MDB strain,with open-reading-frame encoding 219 amino acids.6 expression vectors were constructed based on CP gene,among them,4 could be used to transform via microprojectile bombardment(p35SCPfBar,p35SCPrBar,pUbiCPfBar,and pUbiCPrBar),and 2 used to Agrobacterium tumefaciens-mediated transformation(pCAMIACPf and pCAMBIACPr). |
| Keywords: | maize dwarf mosaic disease sugarcane mosaic virus (SCMV) coat protein(CP) expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |