高效液相色谱法测定栀子苷的含量

建立了一种快速测定栀子及栀子黄中栀子苷含量的高效液相色谱分析方法。采用超声波法提取栀子苷,使用Shimpack HRC-ODS色谱柱(4.6mm×250mm,5μm),以甲醇-水(30:70,V/V)为流动相,采用二极管阵列检测器(检测波长240nm)对栀子苷进行测定。结果表明,采用超声波法提取1.0h可将栀子苷提取完全,在2~24μg/ml范围内栀子苷含量与峰面积呈良好的线性关系,相关系数r=0.9986,精密度和稳定性试验相对标准偏差均小于5%,加样回收率达到98.13%。该测定方法简单、准确、精密度高、重现性好。

DETERMINATION OF GENIPOSIDE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A HPLC method for the rapid determination of geniposide in gardenia and its products was developed.Geniposide was extracted by ultrasonic,separated by the mobile phase of methanol-water on the Shimpack HRC-ODS column,and detected at 240nm by photodiode array detection.The results showed that geniposide could be extracted completely for 1 hour by ultrasonic.Geniposide presented a good linear relation between concentration and peak area within the range of 2～24μg/ml(r=0.9986).The relative standard deviation(RSD) of reproducibility and stability experiments was lower than 5% and the average recovery rate of geniposide was 98.13%,proving that the method was a simple accurate method with high reproducibility.