| 响应面法优化植物乳杆菌产亚油酸异构酶的发酵工艺 |
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| 引用本文： | 孙琪,王振宇,舒适.响应面法优化植物乳杆菌产亚油酸异构酶的发酵工艺[J].东北林业大学学报,2009,37(7). |
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| 作者姓名： | 孙琪 王振宇 舒适 |
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| 作者单位： | 1. 东北林业大学,哈尔滨,150040
2. 贵州大学 |
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| 摘 要： | 采用响应面法对植物乳杆菌的发酵产酶条件进行了优化.选取发酵时间、发酵温度、接种量和底物添加量作为考察因素,在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理,采用4因素3水平的响应面分析法并依据回归分析确定了各工艺条件的影响因子,同时以亚油酸转化率为响应值作响应面和等高线,分析了各个因素的显著性和交互作用.植物乳杆菌产亚油酸异构酶的最佳发酵条件为:发酵时间为30.21 h、发酵温度为36.23 ℃、接种量为1.99%、底物添加量为9.62%.在最佳发酵条件下,实际亚油酸转化率为20.46%.
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| 关 键 词： | 植物乳杆菌 亚油酸异构酶 响应面分析 |
Optimization of Fermentation Conditions for the Production of Linoleate Isomerase from Lactobacillus plantarum Using Response Surface Methodology |
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| Sun Qi,Wang Zhenyu,Shu Shi.Optimization of Fermentation Conditions for the Production of Linoleate Isomerase from Lactobacillus plantarum Using Response Surface Methodology[J].Journal of Northeast Forestry University,2009,37(7). |
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| Authors: | Sun Qi Wang Zhenyu Shu Shi |
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| Institution: | Sun Qi,Wang Zhenyu(College of Forestry,Northeast Forestry University,Harbin 150040,P. R. China),Shu Shi(Guizhou University) |
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| Abstract: | Response surface methodology (RSM) was applied to evaluate the optimum fermentation conditions of Lactobacillus plantarum. Fermentation temperature,fermentation time,inoculation amount,and substrate concentration were chosen as the influencing factors by the single-factor experiments. The optimum conditions were obtained through Box-Behnken center-united experiment design and response surface methodology with 4 factors and 3 levels. Then the factors influencing the technological parameters were determined b... |
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| Keywords: | Lactobacillus plantarum Linoleate isomerase Response surface methodology |
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