利用同源序列发掘抗病候选基因的方法

抗病基因的分离和克隆对于实施作物抗病基因育种及开展抗病机制的研究具有重要意义。目前植物抗病基因的克隆方法主要有转座子标签技术和图位克隆技术。该法对于未知基因产物或者没有进行精细定位的性状来讲，其应用受到极大限制。近年来，很多研究探索了利用同源序列发掘抗病候选基因的途径。本研究归纳和总结了三条发掘抗病候选基因的方法，旨在对作物抗病基因及其连锁标记的发掘起到一定的指导作用。

The Methods of Identifying Resistant Candidate Genes by Homologic Sequences

The isolation and clone of plant disease resistant gene has great significance for plant resistance breeding and the study on disease resistance mechanism. The transposon tagging and map based cloning is the major methods for disease resistance genes cloning in the past. And the use of these two methods are seriously limited for traits with unkown gene product and without fine mapping. Recent studies had shown that resistant candidate genes can be identified by homologic sequences. In this paper, the three methods of identifying resistant candidate genes by using homologic sequeces was expatiated. This will provide some reference for digging disease resistant genes and the linkage markers.