利用ISSR标记研究鹅观草属种质资源的遗传多样性

为进一步了解鹅观草属种质资源的遗传背景和拓宽牧草育种的遗传基础,科学指导鹅观草属种质资源的收集、评价和利用,利用ISSR(Inter simple sequence repeat marker)标记对鹅观草属20个种,6个变种,共60份材料进行了遗传多样性检测。结果发现,被测材料间ISSR标记的多态性较高,在20个引物中,有16个引物可扩增出清晰且重复性好的DNA片段,共产生125条DNA片段,其中104条具有多态性,多态性比率为83.20%;每个引物可扩增出6～10条DNA片段,平均7.81条,材料间遗传相似系数GS变幅为0.188～0.879,平均值为0.375;聚类分析表明,在遗传相似系数为0.441的水平上,60份材料可以聚为5类,属于同种、同组、同系的不同材料首先聚在一起,然后再与其他种的材料聚在一起,此外,材料的聚类还表现出一定的地域性规律。

Genetic Diversity of Roegneria Genera Studied by ISSR Markers

In order to acquire more information about genetic backgrounds on Roegneria and broaden genetic bases for forage breeding,and give more exactly guide on Roegneria germplasm collection,evalution and utilization,genetic diversity of 60 accessions of Roegneria genera representing 20 species and 6 subspecies was evaluated using inter-simple sequence repeat(ISSR) markers in this paper.Twenty ISSR primers were used for the PCR amplification of genomic DNA and 16 primers could produce clear and polymorphic bands.A total of 125 bands were amplified from 16 primers,of which 104 bands(83.20%) were polymorphic.6 to 10 polymorphic bands could be amplified from each primer,with an average 7.81.The mean genetic similarity(GS) value based on ISSR markers among 60 accession of Roegneria genera was 0.375,ranging from 0.188 to 0.879.Cluster analysis showed all materials could be classified into 5 groups,the different accessions belongs to the same species could be clustered together at first,and then combined with other accessions,the accessions from the same origin also could be classified into the same groups first.These results suggested that ISSR markers could be used as an effective molecular technique for forage germplasm diversity research and species identification.