| 苏云金芽胞杆菌CY1菌株的cry基因分析 |
| |
| 引用本文： | 吴会贤,宋萍,王勤英,苏旭东.苏云金芽胞杆菌CY1菌株的cry基因分析[J].华北农学报,2007,22(4):180-183. |
| |
| 作者姓名： | 吴会贤 宋萍 王勤英 苏旭东 |
| |
| 作者单位： | 1. 河北农业大学,植物保护学院,河北省农业病虫害生物防治工程技术研究中心,河北,保定,071001
2. 河北农业大学,食品科技学院,河北,保定,071001 |
| |
| 基金项目： | 河北省自然科学基金项目(303213)，保定市科技局项目(05S001)，河北省农业大学博士基金项目 |
| |
| 摘 要： | 通过醋酸钠-抗生素筛选法从河北省土壤中筛选得到了苏云金芽孢杆菌新菌株CY1。镜检可观察到伴孢晶体呈小菱形。SDS-PAGE分析结果表明菌株CY1表达的晶体蛋白分子量为130 kDa。利用25对通用引物检测cry1,cry1I,cry7,cry2,cry3,cry4/cry10,cry5,cry6,cry7,cry8,cry9,cry11,cry13/14,cry16/17,cry18,cry19,cry21,cry22,cry24/25,cry26/28,cry27/29,cry30,cry32,cry34/35,cry40基因,仅从CY1菌株中检测出cry7基因,该cry7基因N末端的1 215 bp片段所编码的氨基酸序列与已发表的Cry7Ab1(Acc No.:U04367)序列同源性达99%,仅有2个氨基酸的差异。用3对cry7Ab特异引物检测,进一步证实该菌株携带有cry7Ab基因。
|
| 关 键 词： | 苏云金芽胞杆菌 CY1菌株 cry7基因 |
| 文章编号： | 1000-7091（2007）04-0180-04 |
| 修稿时间： | 2007-05-12 |
Characterization of cry Gene from a Novel Bacillus thuringiensis CY1 |
| |
| WU Hui-xian,SONG Ping,WANG Qin-ying,SU Xu-dong.Characterization of cry Gene from a Novel Bacillus thuringiensis CY1[J].Acta Agriculturae Boreali-Sinica,2007,22(4):180-183. |
| |
| Authors: | WU Hui-xian SONG Ping WANG Qin-ying SU Xu-dong |
| |
| Institution: | 1. College of Plant Protection, Agricultural University of Hebei, Biocontrol Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China; 2. College of Food Science and Techhology, Agricultural University of Hebei, Baoding 071001, China |
| |
| Abstract: | A novel strain CY1 of Bacillus thuringiensis(Bt) was isolated from the soil of Hebei province by Sodium acetate-antibiotic method.Bipyramidal inclusions could be visualized by microscope.SDS-PAGE analysis indicated that the strain producing a 130 kDa protein.The cry gene analysis of the strain was based on multiplex PCR with novel general primers that could detect the cry1,cry1I,cry7,cry2,cry3,cry4/cry10,cry5,cry6,cry7,cry8,cry9,cry11,cry13/14,cry16/17,cry18,cry19,cry21,cry22,cry24/25,cry26/28,cry27/29,cry30,cry32,cry34/35 and cry40 genes.Only cry7 gene were found.PCR product of cry7 gene was sequenced.The cry7 gene fragments had 99% homology to cry7Ab1.The strains were further characterized to carry cry7Ab gene by additional PCRs with specific cry7Ab primers. |
| |
| Keywords: | Bacillus thuringiensis CY1 strain cry7 gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
