| 谷子品种遗传差异的RAPD标记分析 |
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| 引用本文： | 杨延兵,管延安,张华文,徐平平,张文兰,陈利容,秦岭.谷子品种遗传差异的RAPD标记分析[J].华北农学报,2007,22(4):134-136. |
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| 作者姓名： | 杨延兵 管延安 张华文 徐平平 张文兰 陈利容 秦岭 |
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| 作者单位： | 1. 山东省农业科学院作物研究所,山东,济南,250100
2. 陕西师范大学,陕西,西安,710062 |
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| 基金项目： | 山东省农业科学院青年科研基金项目资助(2005YQ051)，国家科技支撑计划资助(2006BAD02B02) |
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| 摘 要： | 利用RAPD标记对来自春、夏谷区不同育种单位的23份谷子品种进行了多态性和聚类分析。结果表明,23份品种的RAPD标记的多态性较低,13个引物共扩增出56条多态性带,平均每个引物扩增多态性带为4.31条,引物S30和S45都有7条多态性带,能够鉴别的品种最多为13个。聚类分析表明,当遗传相似性系数为0.75时,23个品种可分为4类,不同生态区的品种可以归为一类,而同一生态区的品种也可以归为不同的类,基于RAPD的遗传相似性和品种来源地没有必然的一致性。
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| 关 键 词： | 谷子 遗传差异 RAPD标记 |
| 文章编号： | 1000-7091（2007）04-0134-03 |
| 修稿时间： | 2007-03-26 |
Genetic Variation Among Varieties of Foxtail Millet( Setaria italica Beauv.) Based on RAPD Markers |
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| YANG Yan-bing,GUAN Yan-an,ZHANG Hua-wen,XU Ping-ping,ZHANG Wen-lan,CHEN Li-rong,QIN Ling.Genetic Variation Among Varieties of Foxtail Millet( Setaria italica Beauv.) Based on RAPD Markers[J].Acta Agriculturae Boreali-Sinica,2007,22(4):134-136. |
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| Authors: | YANG Yan-bing GUAN Yan-an ZHANG Hua-wen XU Ping-ping ZHANG Wen-lan CHEN Li-rong QIN Ling |
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| Institution: | 1. Crop Research Institute, Shandong Academy of Agriculture Science, Jinan 250100, China; 2. Shaanxi Normal University, Xian 710062, China |
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| Abstract: | Genetic variation of 23 varieties of foxtail millet collected from spring millet region and summer millet regions was studied.The results showed that 23 varieties had certain degree of genetic variation in molecular level,but the degree of variation was not significant.13 primers yielded 56 polymorphic bands from 23 varieties and the average of polymorphic bands was 4.31 yielded by each primer.The primers S30 and S45 yielded 7 polymorphic bands,respectively,and which could be used to distinguish 13 varieties.The cluster analysis of RAPD makers indicated that the cluster type of genetic variation of varieties was not consistent with ecological type.Varieties from different regions could be clustered in the same type,and varieties from same regions might be clustered in different types. |
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| Keywords: | Foxtail millet(Setaria Italica Beauv ) Genetic variation RAPD marker |
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