不结球白菜游离小孢子培养成胚影响因素的研究

以17个不同基因型的不结球白菜为供试材料,对影响小孢子胚胎发生频率的若干因素进行了研究。结果表明:在秋冬季日光温室内,供试植株主花序第一朵花开后第7~14天取样,即初花期是最佳取样时期;不同基因型间小孢子胚胎发生能力差异很大,在接种的17个基因型中,有12个诱导出胚,诱导成功率71%;其中102号产胚量最高,达到32.78个/蕾;在已产胚材料中,产胚量最高的基因型是产胚量最低的基因型的546倍;较易抽薹型材料的产胚能力大于易抽薹型和不易抽薹型。以B5有机成分代替NLN有机成分,能够提高产胚量,尤其是对难成胚基因型的小孢子胚胎发生有较好的促进作用,甘露醇对小孢子分裂无促进作用。

Influence Factors to Microspore Embryogenesis of Non-heading Chinese Cabbage

The influence factors to microspore embryogenesis of non-heading Chinese cabbage were studied through seventeen genotypes.The result showed that the best time for sampling was 7 to 14 days after the first bud flowering on the main anthotaxy.The capacity of embryoid production varied significantly in different genotypes.Among them,twelve genotypes yield embryos successfully,which the rate could reach 71%.Genotype 102 was an ideal material for embryoid induction,and the frequency could reach 32.78 embryos per bud.Genotype that with a highest yield produced 546 times as many embryos as that with a lowest yield.The embryoid induction ability of normal bolting genotypes were higher than easy bolting and hard bolting genotypes.The embryoid induction frequency increased when microspores were cultured in medium that supplemented with B5 organic compound instead of NLN organic compound,especially for genotypes that difficult in producing embryos.Mannitol did not contribute to microspore division.