珍贵绢丝昆虫天蚕FXPRLamide家族神经肽的免疫组织化学鉴定

 利用Zooblotting、酶联免疫吸附测定（ELISA）和免疫组织化学等方法，对天蚕的DH、PBAN等FXPRLamide家族神经肽进行鉴定。结果表明，标记的蓖麻蚕的DH-PBAN cDNA片断能够与天蚕、樟蚕、柳蚕和棉铃虫的基因组DNA结合。这证明了天蚕可能存在FXPRLamide家族神经肽中的DH－PBAN基因；天蚕的咽下神经节中FXPRLamide神经肽含量最高，咽下神经节有三簇细胞合成FXPRLamide神经肽，在胸神经节和腹神经节也有明显的阳性细胞；天蚕5龄幼虫的血淋巴中FXPRLamide神经肽含量比较稳定，化蛹前含量升高，蛹期含量降低。上述结果证明天蚕含有DH、PBAN等FXPRLamide家族神经肽，其对天蚕的滞育和性信息素合成等的调控还需要进一步研究。

Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai by Using Immunocytochemistry Methods

In present study, zooblooting, ELISA and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai. The results showed that the genomic DNA from Antheraea yamamai showed positive bands after hybridized with the fragment of DH-PBAN cDNA from Samia cynthia ricini, which labeled with -32P]-dCTP. The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against H. armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of Antheraea yamamai by whole-mount immunocytochemistry, and there were three clusters cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph kept a steady level. When pupation, the titer increased promptly, but decreased to a low level after early pupal stage. The above results demonstrated the existence of FXPRLamide family peptides in Antheraea yamamai, but its function need to be further investigated in the future.