均匀设计优化银杏培养基配比

应用均匀设计法安排银杏体外培养试验.以3、4月份生长旺盛的银杏植株上的幼嫩部位为外植体,包括幼叶、茎段、顶芽,采用MS培养基,添加不同浓度植物生长调节剂2,4-D、6-BA 、NAA,设置6个不同的水平数,按照均匀设计表U*4(64)建立 6个试验组合,运用均匀设计软件分析这些不同材料和不同激素及其各浓度水平配比下的愈伤组织诱导情况及植株的再生效果.通过建立试验方案、数据处理、回归分析以及优化拟合,得到了最佳配方并予以验证.结果表明:以芽为外植体时,最佳的试验配比是NAA 2.0 mg·L-1 6-BA 1.5 mg·L-1 芽,激素2,4-D对试验结果没有显著影响,6-BA、NAA均以浓度适中为宜.

Optimization of Medium Proportion of Ginkgo biloba by Uniform Design

The tissue culture of Ginkgo biloba in different young position,such as leaf,stem section and bud,were carried out in the paper,by using MS medium added different concentrations of plant growth regulators for instance 2,4_D,NAA and 6_BA. Taking uniform design U*-4(64),both the callus induction and the regeneration of explants with different materials and different hormones were analyzed in the experiment. The result showed that the optimal choice was NAA 2.0 mg5L {-1} 6_BA 1.5 mg5L {-1} bud,and there was a lack of significance of difference for 2,4_D.