|
|||||
|
|
| 抗A型产气荚膜梭菌单克隆抗体的研制和鉴定 | |
| 引用本文： | 张红英,杨霞,陈丽颖,王亚宾,金钺,郑杰,卢中华. 抗A型产气荚膜梭菌单克隆抗体的研制和鉴定[J]. 中国畜牧兽医, 2006, 33(9): 62-64 |
| 作者姓名： | 张红英 杨霞 陈丽颖 王亚宾 金钺 郑杰 卢中华 |
| 作者单位： | 河南农业大学,郑州,450002 |
| 摘 要： | 本试验用NSo细胞和产气荚膜梭菌肠毒素免疫的BALB／C小鼠脾细胞融合，获得了2株能分泌抗A型产气荚膜梭菌肠毒索单克隆抗体的杂交瘤细胞。经鉴定．这2株杂交瘤细胞的平均染色体数目为96．8条，单抗亚类为IgG1，杂交瘤细胞培养上清及腹水单克隆抗体的效价均较高，而且与其他几种常见的毒素SE、LT、S—LT、产气荚膜梭菌α毒素等不存在交叉反应。1D5能完全中和NCTC8239、S020718所产生的CPE的生物学活性，而284的中和活性则较差。 |
| 关 键 词： | 产气荚膜梭菌肠毒素 单克隆抗体 |
| 文章编号： | 1671-7236（2006）09-0062-03 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006-01-09 |
Production and Characterization of Monoclonal Antibodies against Clostridium Perfringens Type A Enterotoxin |
|
| ZHANG Hong-ying,YANG Xia,CHENG Li-ying,WANG Ya-bin,JIN Yue,ZHENG Jie,LU Zhong-hua. Production and Characterization of Monoclonal Antibodies against Clostridium Perfringens Type A Enterotoxin[J]. China Animal Husbandry & Veterinary Medicine, 2006, 33(9): 62-64 | |
| Authors: | ZHANG Hong-ying YANG Xia CHENG Li-ying WANG Ya-bin JIN Yue ZHENG Jie LU Zhong-hua |
| Affiliation: | Henan Agricultural University,Zhenzhou 450002,China |
| Abstract: | Hybridomas secreting monoclonal antibodies(McAbs)specific for Clostridium perfringens type A enterotoxin(CPE) were produced by fusion of Nso myelomas cells with spleen cells from BALB/C mice immunized with purified CPE.Wells containing hybridomas secreting immunoglobulin G(IgG) antibodies against CPE were specifically identified by an indirect enzyme-linked immunosorbent assay(ELISA),and two strains of ELISA-positive hybridomas were selected and cloned forth by limited dilution.All two hybridomas produced McAbs containing immunoglobulin G1 heavy chains.These hybridomas were then grown as ascitic tumors in mice,and McAbs were purified from the ascites fluids with DEAE.The specificity of the McAbs was demonstrated by RPLA and ELISA.One of the hybridomas with 96.8 average number of chromosome has the activity of neutralizing CPE. |
| Keywords: | clostridmm perfringens enterotoxin monoclonal antibody |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |