| 海南小黄姜脱毒快繁技术研究 |
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| 引用本文： | 齐 兰,朱红林,陈健晓,白翠云. 海南小黄姜脱毒快繁技术研究[J]. 热带作物学报, 2013, 33(9): 37-40 |
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| 作者姓名： | 齐 兰 朱红林 陈健晓 白翠云 |
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| 作者单位： | 海南省农业科学院粮食作物研究所 海南海口 571100;海南省农业科学院粮食作物研究所 海南海口 571100;海南省农业科学院粮食作物研究所 海南海口 571100;海南省农业科学院粮食作物研究所 海南海口 571100 |
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| 摘 要： | 以海南小黄姜茎尖为材料，进行组培脱毒及离体快繁技术研究，结果表明，剥离茎尖(0.3～0.5 mm)分生组织培养，能有效脱除生姜烟草花叶病毒和黄瓜花叶病毒。筛选出适合丛生芽分化的培养基配方(MS＋6-BA3.0 mg/L＋NAA0.1 mg/L)，实现了生姜增殖与生根诱导同步，增殖系数为8，将试管苗移栽到沙土＋椰糠1:1的混合基质中炼苗，成活率最高为92%。
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| 关 键 词： | 茎尖 ；离体快繁 ；组培苗 ；成活率 |
Virus Elimination and Rapid Propagation of Small Yellow Ginger(Zingiber officinale Rosc) in Hainan |
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| Affiliation: | Cereal Crops Research Institute, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100;Cereal Crops Research Institute, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100;Cereal Crops Research Institute, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100;Cereal Crops Research Institute, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100 |
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| Abstract: | In order to investigate virus elimination and micropropagation of ginger, we use small yellow ginger stem tissue as material. The results showed with stem tip (0.3~0.5 mm) culture, could effectively remove Tobacco mosaic virus and Cucumber mosaic virus of ginger, which showed the medium MS+ 6-BA3.0 mg/L+NAA0.1 mg/L were best for buds propagation, we accomplished the proliferation and root induction synchronised, the propagation coefficient were 8 , transplant to hardening in sand+coconut husk 1:1 mixing matrix had the best transplanting survival rate were 92%. |
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| Keywords: | stem tip in vitro propagation plantlets from tissue culture survival rate |
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