| 基于CRISPR序列的肠道沙门氏菌分离株CSST分型 |
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| 引用本文： | 徐越,张丽霞,王茜月,管飘萍,刘文博.基于CRISPR序列的肠道沙门氏菌分离株CSST分型[J].中国动物检疫,2022,39(3):91-98. |
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| 作者姓名： | 徐越 张丽霞 王茜月 管飘萍 刘文博 |
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| 作者单位： | 扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心 |
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| 基金项目： | 扬州大学大学生科技创新基金项目（X20180616）；江苏省优势学科第三期（2018）资助项目 |
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| 摘 要： | 为验证一种新的CRISPR分型方法——CSST分型方法分型沙门氏菌的可行性,分别采用传统CRISPR与CSST两种分型方法,对20株临床肠道沙门氏菌分离株进行分型.结果显示:CSST分型方法同传统CRISPR分型方法分型沙门氏菌的结果完全一致,均将20株临床肠道沙门氏菌菌株分成了15种亚型,其中CR4/CT4和CR9/...
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| 关 键 词： | 沙门氏菌 沙门氏菌分型 CRISPR序列 CRISPR分型 间隔序列 CSST分型 |
CSST Typing of Salmonella enterica Isolates Based on CRISPR Sequences |
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| Xu Yue,Zhang Lixi,Wang Xiyue,Guan Piaoping,Liu Wenbo.CSST Typing of Salmonella enterica Isolates Based on CRISPR Sequences[J].China Journal Of Animal Quarantine,2022,39(3):91-98. |
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| Authors: | Xu Yue Zhang Lixi Wang Xiyue Guan Piaoping Liu Wenbo |
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| Institution: | (School of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009,China) |
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| Abstract: | In order to verify the feasibility of CSST typing,a new CRISPR typing for Salmonella,20 clinical Salmonella enterica isolates were classified respectively using traditional CRISPR typing and CS ST typing.It was found that the results by the two methods were completely coincident,which classified the 20 isolates into 15 subtypes,in which,CR4/CT4 and CR9/CT9 were the most dominant CR/CT type,while other CR/CT types were sporadically distributed.In conclusion,the CSST typing could be used to type Salmonella enterica and was more simple and convenient than the traditional CRISPR typing.An important technical method was thereby provided for epidemiological traceability of Salmonella. |
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| Keywords: | Salmonella Salmonella typing CRISPR sequence CRISPR typing intervening sequence CSST typing |
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